Cellular mechanisms in ocular immunologically mediated disease are being studied in animal models of experimental autoimmune uveoretinitis (EAU). Rats and mice are immunized with retina-derived antigens or synthetic peptides, representing fragments of these antigens, to induce EAU. Susceptibility to disease induction is being evaluated in various strains o known genetic makeup, in the hope of delineating the hereditary mechanisms that predispose to uveitis. In vivo functional long-term T-cell lines and clones are developed from lymphoid organs of rats and mice immunized with uveitogenic ocular proteins. The functional properties and antigen recepto of these cells are being studied to develop strategies for in vivo targetin of the autoimmune cells. EAU in rats and mice serves as a template for the evaluation of new drugs and compounds as well as for the study and characterization of the participating cells and their factors. The goals o these studies are to identify the immunogenetic factors predisposing to uveitic disease, learn about the pathogenic mechanisms involved, characteri the immunoreactive cells and their mediators, and finally to utilize this knowledge for designing rational approaches to immunotherapy.

Agency
National Institute of Health (NIH)
Institute
National Eye Institute (NEI)
Type
Intramural Research (Z01)
Project #
1Z01EY000184-12
Application #
3755550
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
12
Fiscal Year
1994
Total Cost
Indirect Cost
Name
U.S. National Eye Institute
Department
Type
DUNS #
City
State
Country
United States
Zip Code
St Leger, Anthony J; Hansen, Anna M; Karauzum, Hatice et al. (2018) STAT-3-independent production of IL-17 by mouse innate-like ?? T cells controls ocular infection. J Exp Med 215:1079-1090
Chen, Jun; Caspi, Rachel R; Chong, Wai Po (2018) IL-20 receptor cytokines in autoimmune diseases. J Leukoc Biol 104:953-959
Kielczewski, Jennifer L; Horai, Reiko; Jittayasothorn, Yingyos et al. (2016) Tertiary Lymphoid Tissue Forms in Retinas of Mice with Spontaneous Autoimmune Uveitis and Has Consequences on Visual Function. J Immunol 196:1013-25
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Kyger, Madison; Worley, Aneta; Huan, Jianya et al. (2013) Effective Arrestin-Specific Immunotherapy of Experimental Autoimmune Uveitis with RTL: A Prospect for Treatment of Human Uveitis. Transl Vis Sci Technol 2:1
Hansen, Anna M; Caspi, Rachel R (2009) Targeting lymphotoxin depletes pathogenic T cells. Nat Med 15:732-3

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