The identity and topographic location of ocular inflammatory, degenerative, and tumor cells and their products in patient specimens are analyzed by routine pathology, immunohistochemistry, in situ hybridization, microdissection and various molecular techniques such as PCR, RT-PCR, and RFLP assay. The application of cutting-edge technology allows us to provide accurate pathological assessments of the disease, guides us in selecting an appropriate therapy for the patient, and helps us to better understand the disease mechanisms. In FY2006, we continued and accomplished the following in our research: 1. Detection of Genes and Proteins in Ocular Lymphoma Cells: We reported bcl-2 t(14;18) translocation in 56% of 72 cases with primary intraocular lymphoma (PIOL). Although there was no significant association between bcl-2 t(14;18) translocations and survival or relapse. Patients with translocation were significantly younger. We also reported a relationship between PIOL and testicular lymphoma. We detected infectious genes (H. pylori or C. peumoniae) in conjunctival and orbital MALT lymphoma. 2. Molecular pathology of age-related macular degeneration (AMD): We detected higher rates of single nucleotide polymorphisms and lower expression of CX3CR1 and ERCC6 in the AMD maculae compared to the normal eyes, which confirmed the association studies of these two genes and AMD. 3. New Pathology and Pathogenesis of Ocular Diseases: We discovered stem cell components in ocular hemangioblastomas associated with von Hippel-Lindau disease. We reported ocular pathology of Hermansky-Pudlak syndrome type 1 in an adult whose ocular melanocytes were sparse and contained mainly stage III and rarely stage IV melanosomes. We observed long term survaval of donor epithelial cells after limbal stem cell transplantation, which implied the need of immunosuppresive therapy. We discussed mycobacterium related ocular infalmmatory diseases, and ocular findings in chronic granulomatous disease. 4. Experimental Models for Various Ocular Diseases: We demonstrated exacerbation of retinal degeneration and choroidal neovascularization induced by subretinal injection of matrigel in CCL2/MCP-1 deficient mice, which supported the important role of inflammation played in AMD. In collaboration with Drs. Caspi, Gery, and Nussenblatt, several new modes and mechanisms of ocular inflammation have been discovered and published. In collaboration with Dr. Chew, we found elevated serum inflammatory markers such as ICAM-1, RANTES and CCL2 in patients with diabetic retinopathy.
| Panda, Puneet; Forooghian, Farzin; Goodglick, Todd et al. (2010) Orbital lymphoma masquerading as panuveitis. Ocul Immunol Inflamm 18:181-3 |
| Tuo, Jingsheng; Ross, Robert J; Herzlich, Alexandra A et al. (2009) A high omega-3 fatty acid diet reduces retinal lesions in a murine model of macular degeneration. Am J Pathol 175:799-807 |
| Lu, Ailing; Ng, Lily; Ma, Michelle et al. (2009) Retarded developmental expression and patterning of retinal cone opsins in hypothyroid mice. Endocrinology 150:1536-44 |
| MacDonald, Ian M; Russell, Laurie; Chan, Chi-Chao (2009) Choroideremia: new findings from ocular pathology and review of recent literature. Surv Ophthalmol 54:401-7 |
| Cimino, Luca; Chan, Chi-Chao; Shen, DeFen et al. (2009) Ocular involvement in nasal natural killer T-cell lymphoma. Int Ophthalmol 29:275-9 |
| Fujimoto, C; Klinman, D M; Shi, G et al. (2009) A suppressive oligodeoxynucleotide inhibits ocular inflammation. Clin Exp Immunol 156:528-34 |
| Verma, Varun; Shen, Defen; Sieving, Pamela C et al. (2008) The role of infectious agents in the etiology of ocular adnexal neoplasia. Surv Ophthalmol 53:312-31 |
| Tang, Johnny; Stevens, Rosalind A; Okada, Annabelle A et al. (2008) Association of antiretinal antibodies in acute annular outer retinopathy. Arch Ophthalmol 126:130-2 |
| Ding, Xiaoyan; Herzlich, Alexandra A; Bishop, Rachel et al. (2008) Ocular toxicity of fludarabine: a purine analog. Expert Rev Ophthalmol 3:97-109 |
| Herzlich, Alexandra A; Tuo, Jingsheng; Chan, Chi-Chao (2008) Peroxisome proliferator-activated receptor and age-related macular degeneration. PPAR Res 2008:389507 |
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