Anterior Chamber of the Eye

Russell, P.

Abstract

The trabecular meshwork of th eye is a principal site of outflow resistance in the eye. Initial efforts have shown that the protein compositions of the cells in different quadrants of the trabecular meshwork in a single eye are not significantly different. There is also no discernible difference in meshwork between fellow eyes. Experiments have been started to determine if the cellular protein composition of the trabecular meshwork changes with age. Preliminary indications suggest that difference might be related only to the amount of a specific protein rather than different protein compositions. The trabecular meshwork is stretched in a glaucomatous eye. Trabecular meshwork cells from Rhesus monkeys have been put in tissue culture. These cells as well as a bovine cardiac pulmonary artery endothelial cell line, which serves as a model for Schlemm's canal cells, have been studied under conditions of stretch. The cells grown on silicone sheets have been stretched 10 percent and 20 percent over control conditions. Results indicate a very rapid phosphorylation of specific proteins on tyrosine residues. These proteins as well as subsequent changes in the cell composition are currently being investigated.

Funding Agency

• Agency: National Institute of Health (NIH)
• Institute: National Eye Institute (NEI)
• Type: Intramural Research (Z01)
• Project #: 1Z01EY000303-01
• Application #: 5202357
• Support Year: 1
• Fiscal Year: 1995

Institution

• Name: U.S. National Eye Institute
• Country: United States

Publications

Russell, Paul; Walsh, Erin; Chen, WeiPing et al. (2006) The effect of temperature on gene silencing by siRNAs: implications for silencing in the anterior chamber of the eye. Exp Eye Res 82:1011-6

Sinha, Debasish; Hose, Stacey; Zhang, Cheng et al. (2005) A spontaneous mutation affects programmed cell death during development of the rat eye. Exp Eye Res 80:323-35

Zillig, Markus; Wurm, Antje; Grehn, Franz J et al. (2005) Overexpression and properties of wild-type and Tyr437His mutated myocilin in the eyes of transgenic mice. Invest Ophthalmol Vis Sci 46:223-34

Zhao, Xiujun; Ramsey, Keri E; Stephan, Dietrich A et al. (2004) Gene and protein expression changes in human trabecular meshwork cells treated with transforming growth factor-beta. Invest Ophthalmol Vis Sci 45:4023-34

Rhee, Douglas J; Fariss, Robert N; Brekken, Rolf et al. (2003) The matricellular protein SPARC is expressed in human trabecular meshwork. Exp Eye Res 77:601-7

Rhee, Douglas J; Tamm, Ernst R; Russell, Paul (2003) Donor corneoscleral buttons: a new source of trabecular meshwork for research. Exp Eye Res 77:749-56

van den IJssel, Paul; Wheelock, Robert; Prescott, Alan et al. (2003) Nuclear speckle localisation of the small heat shock protein alpha B-crystallin and its inhibition by the R120G cardiomyopathy-linked mutation. Exp Cell Res 287:249-61

Zigler Jr, J Samuel; Qin, Chuan; Kamiya, Toshikazu et al. (2003) Tempol-H inhibits opacification of lenses in organ culture. Free Radic Biol Med 35:1194-202

Zhao, Xiujun; Pearson, Keri E; Stephan, Dietrich A et al. (2003) Effects of prostaglandin analogues on human ciliary muscle and trabecular meshwork cells. Invest Ophthalmol Vis Sci 44:1945-52

Takase, Hiroshi; Sugita, Sunao; Rhee, Douglas J et al. (2002) The presence of macrophage migration inhibitory factor in human trabecular meshwork and its upregulatory effects on the T helper 1 cytokine. Invest Ophthalmol Vis Sci 43:2691-6