Abstract

The role of the homeobox gene Pax6 in squid development was investigated in collaboration with Dr. P. Callaerts (University of Houston, TX). A detailed expression analysis by in situ hybridization and immunodetection revealed Pax6 mRNA and a protein with predominantly nuclear localization in the developing eye, olfactory organ, brain lobes (optic lobe, olfactory lobe, peduncle lobe, superior frontal lobe and dorsal basal lobe), arms and mantle, suggestive of a role in eye, brain, and sensory organ development. Prox 1 is a marker for lymphatic endothelial cells. Prox 1 specific antibodies were used to demonstrate (in collaboration with Dr. J. Wilting, University of Freiburg, Germany) that superficial lymphatics develop independently from the deep ones and that cells migrating from the lateral somite edge into the limb buds differentiate into three cell populations: myocytes, blood vascular endothelial cells and lymphatic endothelial cells.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Eye Institute (NEI)
Type
Intramural Research (Z01)
Project #
1Z01EY000311-08
Application #
6826701
Study Section
(MMGS)
Project Start
Project End
Budget Start
Budget End
Support Year
8
Fiscal Year
2003
Total Cost
Indirect Cost

  Institution

Name
U.S. National Eye Institute
Department
Type
DUNS #
City
State
Country
United States
Zip Code

  Publications

Nakaya, Naoki; Sultana, Afia; Munasinghe, Jeeva et al. (2013) Deletion in the N-terminal half of olfactomedin 1 modifies its interaction with synaptic proteins and causes brain dystrophy and abnormal behavior in mice. Exp Neurol 250:205-18
Nakaya, Naoki; Lee, Hee-Sheung; Takada, Yuichiro et al. (2008) Zebrafish olfactomedin 1 regulates retinal axon elongation in vivo and is a modulator of Wnt signaling pathway. J Neurosci 28:7900-10
Lee, Hee-Sheung; Tomarev, Stanislav I (2007) Optimedin induces expression of N-cadherin and stimulates aggregation of NGF-stimulated PC12 cells. Exp Cell Res 313:98-108
Galeeva, A; Treuter, E; Tomarev, S et al. (2007) A prospero-related homeobox gene Prox-1 is expressed during postnatal brain development as well as in the adult rodent brain. Neuroscience 146:604-16
Nakaya, Naoki; Tomarev, Stanislav (2007) Expression patterns of alternative transcripts of the zebrafish olfactomedin 1 genes. Gene Expr Patterns 7:723-9
Wilting, Jorg; Aref, Yama; Huang, Ruijin et al. (2006) Dual origin of avian lymphatics. Dev Biol 292:165-73
Grinchuk, Oleg; Kozmik, Zbynek; Wu, Xiaofang et al. (2005) The Optimedin gene is a downstream target of Pax6. J Biol Chem 280:35228-37
Reis, Rui M; Reis-Filho, Jorge S; Longatto Filho, Adhemar et al. (2005) Differential Prox-1 and CD 31 expression in mucousae, cutaneous and soft tissue vascular lesions and tumors. Pathol Res Pract 201:771-6
Murphy, Patrick J M; Morishima, Yoshihiro; Kovacs, Jeffrey J et al. (2005) Regulation of the dynamics of hsp90 action on the glucocorticoid receptor by acetylation/deacetylation of the chaperone. J Biol Chem 280:33792-9
Steffensen, Knut R; Holter, Elin; Bavner, Ann et al. (2004) Functional conservation of interactions between a homeodomain cofactor and a mammalian FTZ-F1 homologue. EMBO Rep 5:613-9

Showing the most recent 10 out of 21 publications