Research in the Unit on Neurocytology and Physiology, is concerned with understanding how the brain develops and modifies its structure and function through experience. Functional activity in the brain during late stages of fetal development and in early postnatal life is essential for normal development of the nervous system of higher vertebrates. Our research is investigating the molecular mechanisms that enable neural impulse activity to regulate major developmental processes of both neurons and glia. This main objectives of this research program are: (1) to understand how the expression of genes controlling the developing structure and function of the nervous system are regulated by patterned neural impulse activity; (2) to determine the functional consequences of neural impulse activity on major developmental processes, including: cell proliferation, survival, differentiation, growth cone motility, axon bundling (fasciculation), neurite outgrowth, synaptogenesis and synapse remodeling, myelination, interactions with glia, and the mechanisms of learning and memory in postnatal animals; (3) to understand how information contained in the temporal pattern of neural impulse activity is transduced and integrated within the intracellular signaling networks of neurons to activate specific genes and control appropriate adaptive responses. Major achievements of research in the last year include: (1) the discovery that development of glia of the peripheral nervous system (Schwann cells) is regulated by neural impulse activity in premyelinated neurons, and determined that extracellular ATP is the signaling molecule communicating neural impulse activity from neurons to glia; (2) tested the hypothesis that a key signaling protein activated by neural impulse activity, calcium-calmodulin dependent protein kinase II (CaM KII) can decode different frequencies of action potentials by autophosphorylation at Thr-286; (3) determined that different calcium-dependent signaling pathways are activated in CA1 neurons by different stimulus patterns to phosphorylate the signaling molecule MAPK in association with long-term potentiation (LTP) in the hippocampus.

Project Start
Project End
Budget Start
Budget End
Support Year
6
Fiscal Year
2000
Total Cost
Indirect Cost
Name
U.S. National Inst/Child Hlth/Human Dev
Department
Type
DUNS #
City
State
Country
United States
Zip Code
Bukalo, Olena; Lee, Philip R; Fields, R Douglas (2016) BDNF mRNA abundance regulated by antidromic action potentials and AP-LTD in hippocampus. Neurosci Lett 635:97-102
Lee, Philip R; Fields, R Douglas (2009) Regulation of myelin genes implicated in psychiatric disorders by functional activity in axons. Front Neuroanat 3:4
Fields, R Douglas (2008) Oligodendrocytes changing the rules: action potentials in glia and oligodendrocytes controlling action potentials. Neuroscientist 14:540-3
Fields, R Douglas (2008) White matter in learning, cognition and psychiatric disorders. Trends Neurosci 31:361-70
Fields, R Douglas (2008) White matter matters. Sci Am 298:42-9
Jia, Min; Li, Min-Xu; Fields, R Douglas et al. (2007) Extracellular ATP in activity-dependent remodeling of the neuromuscular junction. Dev Neurobiol 67:924-32
Fields, R Douglas; Fields, Kyle D; Fields, Melanie C (2007) Semiconductor gel in shark sense organs? Neurosci Lett 426:166-70
Lee, Philip R; Cohen, Jonathan E; Fields, R Douglas (2006) Immune system evasion by peripheral nerve sheath tumor. Neurosci Lett 397:126-9
Fields, R Douglas (2006) Nerve impulses regulate myelination through purinergic signalling. Novartis Found Symp 276:148-58; discussion 158-61, 233-
Cohen, Jonathan E; Fields, R Douglas (2006) CaMKII inactivation by extracellular Ca(2+) depletion in dorsal root ganglion neurons. Cell Calcium 39:445-54

Showing the most recent 10 out of 29 publications