Three-Dimensional Structures of Biological Macromolecules

Ferretti, J A.

Abstract

Solution and solid state nuclear magnetic resonance spectroscopy are being used along with circular dichroism, microelectrophoretic mobility, fluorescence spectroscopy, differential scanning calorimetry, mass spectrometry and computer modelling to study the structure of peptides and small proteins in solution and in the bound state. Proteins which have been investigated include the homeodomain (DNA binding domain) of the NK-2 protein encoded from the homeobox gene of Drosophila melanogaster and the third variable domain of gp120 from HIV-1. Studies are also in progress on an antigenic peptide bound to a monoclonal antibody and a peptide epitope bound to major histocompatibility complex.

Funding Agency

• Agency: National Institute of Health (NIH)
• Institute: National Heart, Lung, and Blood Institute (NHLBI)
• Type: Intramural Research (Z01)
• Project #: 1Z01HL001027-10
• Application #: 3843280
• Support Year: 10
• Fiscal Year: 1992

Institution

• Name: National Heart, Lung, and Blood Institute
• Country: United States

Publications

Wu, Xiongwu; Pickard 4th, Frank C; Brooks, Bernard R (2016) Isotropic periodic sum for multipole interactions and a vector relation for calculation of the Cartesian multipole tensor. J Chem Phys 145:164110

Rinschen, Markus M; Bharill, Puneet; Wu, Xiongwu et al. (2016) The ubiquitin ligase Ubr4 controls stability of podocin/MEC-2 supercomplexes. Hum Mol Genet 25:1328-44

Roubinian, Nareg H; Looney, Mark R; Kor, Daryl J et al. (2015) Cytokines and clinical predictors in distinguishing pulmonary transfusion reactions. Transfusion 55:1838-46

Wu, Xiongwu; Brooks, Bernard R (2015) A Virtual Mixture Approach to the Study of Multistate Equilibrium: Application to Constant pH Simulation in Explicit Water. PLoS Comput Biol 11:e1004480

Wu, Xiongwu; Subramaniam, Sriram; Case, David A et al. (2013) Targeted conformational search with map-restrained self-guided Langevin dynamics: application to flexible fitting into electron microscopic density maps. J Struct Biol 183:429-440

Singh, Rishi P; Brooks, Bernard R; Klauda, Jeffery B (2009) Binding and release of cholesterol in the Osh4 protein of yeast. Proteins 75:468-77

Lengyel, Jeffrey S; Stott, Katherine M; Wu, Xiongwu et al. (2008) Extended polypeptide linkers establish the spatial architecture of a pyruvate dehydrogenase multienzyme complex. Structure 16:93-103

Hwang, Kae-Jung; Mahmoodian, Fatemeh; Ferretti, James A et al. (2007) Intramolecular interaction in the tail of Acanthamoeba myosin IC between the SH3 domain and a putative pleckstrin homology domain. Proc Natl Acad Sci U S A 104:784-9

Ju, Jeong Ho; Maeng, Jin-Soo; Zemedkun, Micheas et al. (2006) Physical and functional interactions between the prostate suppressor homeoprotein NKX3.1 and serum response factor. J Mol Biol 360:989-99

Milne, Jacqueline L S; Wu, Xiongwu; Borgnia, Mario J et al. (2006) Molecular structure of a 9-MDa icosahedral pyruvate dehydrogenase subcomplex containing the E2 and E3 enzymes using cryoelectron microscopy. J Biol Chem 281:4364-70