The organization of the apical membrane of cultured kidney cells (MDCK) has been studied by high-resolution light microscopy. The distribution of lipids and proteins has been determined by labelling with fluorescent dyes. Two proteins have been identified on the apical membrane surface and their distribution has been characterized as a function of cell development. The primary cilium has been shown to function as a flow sensor and to play a crucial role in the genesis of autosomal dominant polycystic kidney disease.
| Praetorius, Jeppe; Spring, Kenneth R (2002) Specific lectins map the distribution of fibronectin and beta 1-integrin on living MDCK cells. Exp Cell Res 276:52-62 |
| Praetorius, H A; Spring, K R (2001) Bending the MDCK cell primary cilium increases intracellular calcium. J Membr Biol 184:71-9 |
| Spring, K R (2000) Scientific imaging with digital cameras. Biotechniques 29:70-2, 74, 76 |
| Kovbasnjuk, O N; Bungay, P M; Spring, K R (2000) Diffusion of small solutes in the lateral intercellular spaces of MDCK cell epithelium grown on permeable supports. J Membr Biol 175:16-Sep |
| Kovbasnjuk, O N; Spring, K R (2000) The apical membrane glycocalyx of MDCK cells. J Membr Biol 176:19-29 |
