The kidney contains several distinct epithelia that, in their aggregate function, are responsible for formation of the urine and the maintenance of fluid and electrolyte homeostasis. We are studying the roles of these epithelia in the regulation of the excretion of water, urea, sodium, and chloride. The two central approaches are 1)to microdissect the epithelia from the kidney and to study their functions in vitro, and 2) to make antibodies to each of the cloned transporters expressed along the renal tubule to allow direct study of the regulation of the transporters. The studies measure the transport by the epithelia, the level of expression of various transport proteins in renal tubules, intracellular signaling in individual renal tubule segments, and the levels of nucleic acids (mRNA) for proteins that are involved in the regulation of both the transport and metabolic functions of the epithelia. The current focus is on: 1) mechanisms of NaCl retention in hypertension and in extracellular fluid volume expanded states (congestive heart failure, cirrhosis, nephrotic syndrome); 2) the molecular physiology of the urinary concentrating mechanism; 3)development and characterization of polyclonal antibodies against renal transporter and channel proteins; 4) molecular physiology of renal NaCl transport regulation including regulation of the epithelial sodium channel (ENaC) by vasopressin and aldosterone.
| Saethang, Thammakorn; Hodge, Kenneth; Kimkong, Ingorn et al. (2018) AbDesigner3D: a structure-guided tool for peptide-based antibody production. Bioinformatics 34:2158-2160 |
| LeMaire, Sophia M; Raghuram, Viswanathan; Grady, Cameron R et al. (2017) Serine/threonine phosphatases and aquaporin-2 regulation in renal collecting duct. Am J Physiol Renal Physiol 312:F84-F95 |
| Sandoval, Pablo C; Claxton, J'Neka S; Lee, Jae Wook et al. (2016) Systems-level analysis reveals selective regulation of Aqp2 gene expression by vasopressin. Sci Rep 6:34863 |
| Cheng, Lei; Pisitkun, Trairak; Knepper, Mark A et al. (2016) Peptide Labeling Using Isobaric Tagging Reagents for Quantitative Phosphoproteomics. Methods Mol Biol 1355:53-70 |
| Sanghi, Akshay; Zaringhalam, Matthew; Corcoran, Callan C et al. (2014) A knowledge base of vasopressin actions in the kidney. Am J Physiol Renal Physiol 307:F747-55 |
| Bradford, Davis; Raghuram, Viswanathan; Wilson, Justin L L et al. (2014) Use of LC-MS/MS and Bayes' theorem to identify protein kinases that phosphorylate aquaporin-2 at Ser256. Am J Physiol Cell Physiol 307:C123-39 |
| Knepper, Mark A; Raghuram, Viswanathan; Bradford, Davis et al. (2014) Letter to the editor: ""Systems biology versus reductionism in cell physiology"". Am J Physiol Cell Physiol 307:C308-9 |
| Hoffert, Jason D; Pisitkun, Trairak; Saeed, Fahad et al. (2014) Global analysis of the effects of the V2 receptor antagonist satavaptan on protein phosphorylation in collecting duct. Am J Physiol Renal Physiol 306:410-21 |
| Trepiccione, Francesco; Pisitkun, Trairak; Hoffert, Jason D et al. (2014) Early targets of lithium in rat kidney inner medullary collecting duct include p38 and ERK1/2. Kidney Int 86:757-67 |
| Saeed, Fahad; Hoffert, Jason D; Knepper, Mark A (2014) CAMS-RS: Clustering Algorithm for Large-Scale Mass Spectrometry Data Using Restricted Search Space and Intelligent Random Sampling. IEEE/ACM Trans Comput Biol Bioinform 11:128-41 |
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