Retroviral Mediated Gene Transfer Into Primate Hematopoietic Cells

Donahue, R.

Abstract

The rhesus monkeys have been used to access a gene transduction efficiency in non-human primate model. Pseudotyping using the vesicular stomatitis virus G envelope protein has not increased transduction efficiency. CD4-enriched lymphocytes transduced with a retroviral vector that incorporates the gibbon ape leukemia virus envelope protein express aTat/Rev gene and are associated with reduced pathogenic events normally observed during early simian immuno-deficiency virus infection.

Funding Agency

• Agency: National Institute of Health (NIH)
• Institute: National Heart, Lung, and Blood Institute (NHLBI)
• Type: Intramural Research (Z01)
• Project #: 1Z01HL002338-07
• Application #: 6162708
• Study Section: Special Emphasis Panel (HB)
• Support Year: 7
• Fiscal Year: 1997

Institution

• Name: National Heart, Lung, and Blood Institute
• Country: United States

Publications

An, D S; Kung, S K; Bonifacino, A et al. (2001) Lentivirus vector-mediated hematopoietic stem cell gene transfer of common gamma-chain cytokine receptor in rhesus macaques. J Virol 75:3547-55

Sellers, S E; Tisdale, J F; Agricola, B A et al. (2001) The effect of multidrug-resistance 1 gene versus neo transduction on ex vivo and in vivo expansion of rhesus macaque hematopoietic repopulating cells. Blood 97:1888-91

Takatoku, M; Sellers, S; Agricola, B A et al. (2001) Avoidance of stimulation improves engraftment of cultured and retrovirally transduced hematopoietic cells in primates. J Clin Invest 108:447-55

Donahue, R E; Sorrentino, B P; Hawley, R G et al. (2001) Fibronectin fragment CH-296 inhibits apoptosis and enhances ex vivo gene transfer by murine retrovirus and human lentivirus vectors independent of viral tropism in nonhuman primate CD34+ cells. Mol Ther 3:359-67

Donahue, R E; Dunbar, C E (2001) Update on the use of nonhuman primate models for preclinical testing of gene therapy approaches targeting hematopoietic cells. Hum Gene Ther 12:607-17

Dunbar, C E; Takatoku, M; Donahue, R E (2001) The impact of ex vivo cytokine stimulation on engraftment of primitive hematopoietic cells in a non-human primate model. Ann N Y Acad Sci 938:236-44; discussion 244-5

Heim, D A; Hanazono, Y; Giri, N et al. (2000) Introduction of a xenogeneic gene via hematopoietic stem cells leads to specific tolerance in a rhesus monkey model. Mol Ther 1:533-44

Handa, A; Dickstein, B; Young, N S et al. (2000) Prevalence of the newly described human circovirus, TTV, in United States blood donors. Transfusion 40:245-51

Handa, A; Jubran, R F; Dickstein, B et al. (2000) GB virus C/Hepatitis G virus infection is frequent in American children and young adults. Clin Infect Dis 30:569-71

Wu, T; Kim, H J; Sellers, S E et al. (2000) Prolonged high-level detection of retrovirally marked hematopoietic cells in nonhuman primates after transduction of CD34+ progenitors using clinically feasible methods. Mol Ther 1:285-93