Abstract

Computer analysis, including application of several new algorithms, have been carried out in close collaboration with experimental laboratories, and new computational strategies have been developed in response to questions arising about the structures, functions, interactions and genetics of the proteins of interest. Several important parasites and pathogens, including the malaria parasite Plasmodium falciparum, show extreme bias in the residue compositions of both genes and proteins. These compositional properties, together with questions about the roles of low-complexity, nonglobular and conformationally mobile parts of proteins, are relevant to aspects of the pathogenicity of these organisms and their interactions with the mammalian hosts, with applications to vaccine design and the development of new insights into drug resistance. - Computer algorithms, protein sequences, genome sequences, parasites, bacteria, archaea, compositional bias

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Library of Medicine (NLM)
Type
Intramural Research (Z01)
Project #
1Z01LM000026-08
Application #
6290482
Study Section
Special Emphasis Panel (CBB)
Project Start
Project End
Budget Start
Budget End
Support Year
8
Fiscal Year
1999
Total Cost
Indirect Cost

  Institution

Name
National Library of Medicine
Department
Type
DUNS #
City
State
Country
United States
Zip Code

  Publications

Taylor, S; Barragan, A; Su, C et al. (2006) A secreted serine-threonine kinase determines virulence in the eukaryotic pathogen Toxoplasma gondii. Science 314:1776-80
Khan, Asis; Taylor, Sonya; Su, Chunlei et al. (2005) Composite genome map and recombination parameters derived from three archetypal lineages of Toxoplasma gondii. Nucleic Acids Res 33:2980-92
Su, Xin-zhuan; Wootton, John C (2004) Genetic mapping in the human malaria parasite Plasmodium falciparum. Mol Microbiol 53:1573-82
Loyevsky, Mark; Mompoint, Farah; Yikilmaz, Emine et al. (2003) Expression of a recombinant IRP-like Plasmodium falciparum protein that specifically binds putative plasmodial IREs. Mol Biochem Parasitol 126:231-8
Mu, Jianbing; Ferdig, Michael T; Feng, Xiaorong et al. (2003) Multiple transporters associated with malaria parasite responses to chloroquine and quinine. Mol Microbiol 49:977-89
Takala, Shannon; Branch, OraLee; Escalante, Ananias A et al. (2002) Evidence for intragenic recombination in Plasmodium falciparum: identification of a novel allele family in block 2 of merozoite surface protein-1: Asembo Bay Area Cohort Project XIV. Mol Biochem Parasitol 125:163-71
Wootton, John C; Feng, Xiaorong; Ferdig, Michael T et al. (2002) Genetic diversity and chloroquine selective sweeps in Plasmodium falciparum. Nature 418:320-3
Deitsch, K W; Carlton, J M; Wootton, J C et al. (2001) Host sequences in Plasmodium falciparum and Plasmodium vivax genomic DNA: horizontal transfer or contamination artifact? FEBS Lett 491:164-5
Nomura, T; Carlton, J M; Baird, J K et al. (2001) Evidence for different mechanisms of chloroquine resistance in 2 Plasmodium species that cause human malaria. J Infect Dis 183:1653-61
Fidock, D A; Nomura, T; Talley, A K et al. (2000) Mutations in the P. falciparum digestive vacuole transmembrane protein PfCRT and evidence for their role in chloroquine resistance. Mol Cell 6:861-71

Showing the most recent 10 out of 11 publications