An external atmospheric pressure electrospray ionization source and electrostatic lenses for ion transport which was designed and constructed for an ion cyclotron resonance spectrometer has been improved by the addition of deflection plates. Picoampere ion currents have been trapped and accumulated with the result that high mass resolution for electrosprayed ions of 5-15,000 dalton molecular weight has been demonstrated. Deflection plates increase the effective duty cycle of the spectrometer, with a resulting important enhancement in signal intensity. Further improvements in the electrospray source and the ion cyclotron resonance spectrometer are anticipated, so that the resulting system can be used to measure the molecular weights of proteins and DNA fragments. Derivatization chemistry and separation conditions suitable for the detection of kynurenic acid and metabolites of cholorotryptophan analogs (indoleamine dioxygenase inhibitors) have been demonstrated using liquid chromatography-particle beam-negative chemical ionization mass spectrometry. Several derivatization reagents (substituted phenyl boronic acids) have been tested for use in forming cyclic boronates of glycols for improved detection in negative chemical ionization mass spectrometry. Organic ion imaging using tandem mass spectrometry has been refined so that spatially resolved mass selective images, representing a type of molecular or chemical microscopy can now be generated from non-conducting surfaces.
| Nanavati, Dhaval; Austin, Daniel R; Catapano, Lisa A et al. (2011) The effects of chronic treatment with mood stabilizers on the rat hippocampal post-synaptic density proteome. J Neurochem 119:617-29 |
| Lockwich, Timothy; Pant, Jaya; Makusky, Anthony et al. (2008) Analysis of TRPC3-interacting proteins by tandem mass spectrometry. J Proteome Res 7:979-89 |
| Turck, Christoph W; Falick, Arnold M; Kowalak, Jeffrey A et al. (2007) The Association of Biomolecular Resource Facilities Proteomics Research Group 2006 study: relative protein quantitation. Mol Cell Proteomics 6:1291-8 |
| Tuo, Jingsheng; Bojanowski, Christine M; Zhou, Min et al. (2007) Murine ccl2/cx3cr1 deficiency results in retinal lesions mimicking human age-related macular degeneration. Invest Ophthalmol Vis Sci 48:3827-36 |
| Arnott, David; Gawinowicz, Mary Ann; Kowalak, Jeffrey A et al. (2007) ABRF-PRG04: differentiation of protein isoforms. J Biomol Tech 18:124-34 |
| Glasker, Sven; Vortmeyer, Alexander O; Lonser, Russell R et al. (2006) Proteomic analysis of hemangioblastoma cyst fluid. Cancer Biol Ther 5:549-53 |
| Fujigaki, Hidetsugu; Saito, Kuniaki; Lin, Felix et al. (2006) Nitration and inactivation of IDO by peroxynitrite. J Immunol 176:372-9 |
| Zhuang, Zhengping; Huang, Steve; Kowalak, Jeff A et al. (2006) From tissue phenotype to proteotype: sensitive protein identification in microdissected tumor tissue. Int J Oncol 28:103-10 |
| Laurent, Claudine; Levinson, Douglas F; Schwartz, Sarah A et al. (2005) Direct profiling of the cerebellum by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry: A methodological study in postnatal and adult mouse. J Neurosci Res 81:613-21 |
| Xu, Ming; Geer, Lewis Y; Bryant, Stephen H et al. (2005) Assessing data quality of peptide mass spectra obtained by quadrupole ion trap mass spectrometry. J Proteome Res 4:300-5 |
Showing the most recent 10 out of 20 publications
