Regional Cerebral Circulation and Metabolism

Sokoloff, L.

Abstract

The deoxyglucose method for the quantitative determination of rates of local glucose consumption in the discrete functional and structural components of the brain of conscious or anesthetized laboratory animals was developed in this laboratory over 10 years ago. In this method (14C)deoxyglucose is employed as a tracer for glucose flux through the hexokinase step; the product, (14C)deoxyglucose-6-phosphate, is measured by quantitative autoradiography. The method continues to be used to study alterations in local energy metabolism in a variety of physiological, pharmacological and a limited number of pathological states. Its suitability to a wider range of pathologic conditions is being extended and special time constraints which may be present in the method's adaptation for use in human subjects with (18C)fluorodeoxyglucose and PET have been examined.

Funding Agency

• Agency: National Institute of Health (NIH)
• Institute: National Institute of Mental Health (NIMH)
• Type: Intramural Research (Z01)
• Project #: 1Z01MH000882-21
• Application #: 3944659
• Support Year: 21
• Fiscal Year: 1987

Institution

• Name: U.S. National Institute of Mental Health
• Country: United States

Publications

Poremba, Amy; Saunders, Richard C; Crane, Alison M et al. (2003) Functional mapping of the primate auditory system. Science 299:568-72

Itoh, Yoshiaki; Esaki, Takanori; Shimoji, Kazuaki et al. (2003) Dichloroacetate effects on glucose and lactate oxidation by neurons and astroglia in vitro and on glucose utilization by brain in vivo. Proc Natl Acad Sci U S A 100:4879-84

Esaki, Takanori; Suzuki, Hideyo; Cook, Michelle et al. (2003) Functional activation of cerebral metabolism in mice with mutated thyroid hormone nuclear receptors. Endocrinology 144:4117-22

Esaki, Takanori; Itoh, Yoshiaki; Shimoji, Kazuaki et al. (2002) Effects of dopamine receptor blockade on cerebral blood flow response to somatosensory stimulation in the unanesthetized rat. J Pharmacol Exp Ther 303:497-502

Cohen, David M; Wei, Jingna; O'Brian Smith, E et al. (2002) A method for measuring cerebral glucose metabolism in vivo by 13C-NMR spectroscopy. Magn Reson Med 48:1063-7

Esaki, Takanori; Itoh, Yoshiaki; Shimoji, Kazuaki et al. (2002) Blockade of K(ATP) channels with glibenclamide does not alter functional activation of cerebral blood flow in the unanesthetized rat. Brain Res 948:56-63

Itoh, Y; Esaki, T; Cook, M et al. (2001) Local and global cerebral blood flow and glucose utilization in the alpha-galactosidase A knockout mouse model of Fabry disease. J Neurochem 79:1217-24

Gotoh, J; Itoh, Y; Kuang, T Y et al. (2000) Negligible glucose-6-phosphatase activity in cultured astroglia. J Neurochem 74:1400-8

Itoh, Y; Law, M J; Sokoloff, L (2000) Effects of the Na(+)/H(+) exchanger monensin on intracellular pH in astroglia. Brain Res 882:226-9

Turkheimer, F; Pettigrew, K; Sokoloff, L et al. (2000) Selection of an adaptive test statistic for use with multiple comparison analyses of neuroimaging data. Neuroimage 12:219-29