This project is directed toward the development, validation, and application of methods for measurement of physiological and biochemical processes in functional and structural regions of the nervous system. The methods are first designed for use in animals with quantitative autoradiography and then adapted for use in man with PET. The [14C]iodoantipyrine ([14C]IAP) method for measuring local cerebral blood flow (1CBF) and the [14C]deoxyglucose ([14C]DG) method for measuring local cerebral glucose utilization (1CMR-glc) were developed in this program; adaptations of these methods are the only generally accepted quantitative methods routinely used in man with PET. These methods are applied to various physiological, pharmacological, and pathological states. Efforts this past year have been directed toward these goals: 1) evaluation of necessary parameters to apply the DG method to pathological states when the values of these parameters may deviate from normal; 2) development of a sequential double label procedure to study control and altered behavioral states in the same animal with the autoradiographic methods; 3) model development and evaluation to improve accuracy and reliability of the [18F]fluorodeoxyglucose ([18F]FDG) adaptation of the (14C]DG method for human use with PET.

Agency
National Institute of Health (NIH)
Institute
National Institute of Mental Health (NIMH)
Type
Intramural Research (Z01)
Project #
1Z01MH000882-25
Application #
3845182
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
25
Fiscal Year
1992
Total Cost
Indirect Cost
Name
U.S. National Institute of Mental Health
Department
Type
DUNS #
City
State
Country
United States
Zip Code
Poremba, Amy; Saunders, Richard C; Crane, Alison M et al. (2003) Functional mapping of the primate auditory system. Science 299:568-72
Itoh, Yoshiaki; Esaki, Takanori; Shimoji, Kazuaki et al. (2003) Dichloroacetate effects on glucose and lactate oxidation by neurons and astroglia in vitro and on glucose utilization by brain in vivo. Proc Natl Acad Sci U S A 100:4879-84
Esaki, Takanori; Suzuki, Hideyo; Cook, Michelle et al. (2003) Functional activation of cerebral metabolism in mice with mutated thyroid hormone nuclear receptors. Endocrinology 144:4117-22
Esaki, Takanori; Itoh, Yoshiaki; Shimoji, Kazuaki et al. (2002) Effects of dopamine receptor blockade on cerebral blood flow response to somatosensory stimulation in the unanesthetized rat. J Pharmacol Exp Ther 303:497-502
Cohen, David M; Wei, Jingna; O'Brian Smith, E et al. (2002) A method for measuring cerebral glucose metabolism in vivo by 13C-NMR spectroscopy. Magn Reson Med 48:1063-7
Esaki, Takanori; Itoh, Yoshiaki; Shimoji, Kazuaki et al. (2002) Blockade of K(ATP) channels with glibenclamide does not alter functional activation of cerebral blood flow in the unanesthetized rat. Brain Res 948:56-63
Itoh, Y; Esaki, T; Cook, M et al. (2001) Local and global cerebral blood flow and glucose utilization in the alpha-galactosidase A knockout mouse model of Fabry disease. J Neurochem 79:1217-24
Gotoh, J; Itoh, Y; Kuang, T Y et al. (2000) Negligible glucose-6-phosphatase activity in cultured astroglia. J Neurochem 74:1400-8
Itoh, Y; Law, M J; Sokoloff, L (2000) Effects of the Na(+)/H(+) exchanger monensin on intracellular pH in astroglia. Brain Res 882:226-9
Turkheimer, F; Pettigrew, K; Sokoloff, L et al. (2000) Selection of an adaptive test statistic for use with multiple comparison analyses of neuroimaging data. Neuroimage 12:219-29

Showing the most recent 10 out of 15 publications