Viral and Cellular Factors Governing Retroviral Infection

Eiden, M V.

Abstract

Hamster cells are resistant to infection by most retroviruses including Moloney murine leukemia virus (MoMLV), and gibbon ape leukemia viruses (GaLVs). We have constructed GaLV/MoMLV hybrid recombinant virions to identify viral and cellular determinants responsible for the inability of GaLV and MoMLV to infect hamster cells. The substitution of MoMLV core components for GaLV core components circumvents the resistance of hamster cells to infection by GaLV, demonstrating that hamster cells have receptors for GaLV but are not efficiently infected by this primate retrovirus due to a post-penetration block. In contrast, the resistance of hamster cells to MoMLV infection is apparently due to the presence of a nonfunctional viral MoMLV receptor. Treatment of CHO K1 or BHK 21 cells with the glycosylation inhibitor tunicamycin allows these cells to be infected by MoMLV. The construction of recombinant MoMLV/GaLV hybrid virions that can efficiently infect resistant cells has allowed the identification of viral and cellular factors responsible for restricting productive infection of hamster cells by MoMLV and GaLV suggest specific chemical and genetic modifications of target cells that facilitate MoMLV infection.

Funding Agency

• Agency: National Institute of Health (NIH)
• Institute: National Institute of Mental Health (NIMH)
• Type: Intramural Research (Z01)
• Project #: 1Z01MH002592-01
• Application #: 3860037
• Support Year: 1
• Fiscal Year: 1991

Institution

• Name: U.S. National Institute of Mental Health
• Country: United States

Publications

Emery, Andrew C; Xu, Wenqin; Eiden, Maribeth V et al. (2017) Guanine nucleotide exchange factor Epac2-dependent activation of the GTP-binding protein Rap2A mediates cAMP-dependent growth arrest in neuroendocrine cells. J Biol Chem 292:12220-12231

Emery, Andrew C; Alvarez, Ryan A; Abboud, Philip et al. (2016) C-terminal amidation of PACAP-38 and PACAP-27 is dispensable for biological activity at the PAC1 receptor. Peptides 79:39-48

Farrell, Karen B; Tusnady, Gabor E; Eiden, Maribeth V (2009) New structural arrangement of the extracellular regions of the phosphate transporter SLC20A1, the receptor for gibbon ape leukemia virus. J Biol Chem 284:29979-87

Oliveira, Nidia M; Satija, Harshita; Kouwenhoven, I Arlette et al. (2007) Changes in viral protein function that accompany retroviral endogenization. Proc Natl Acad Sci U S A 104:17506-11

Oliveira, Nidia M; Farrell, Karen B; Eiden, Maribeth V (2006) In vitro characterization of a koala retrovirus. J Virol 80:3104-7

Gemeniano, Malou; Mpanju, Onesmo; Salomon, Daniel R et al. (2006) The infectivity and host range of the ecotropic porcine endogenous retrovirus, PERV-C, is modulated by residues in the C-terminal region of its surface envelope protein. Virology 346:108-17

Farrell, Karen B; Eiden, Maribeth V (2005) Dissection of gammaretroviral receptor function by using type III phosphate transporters as models. J Virol 79:9332-6

Wang, Wei; Jobbagy, Zsolt; Bird, Terry H et al. (2005) Cell signaling through the protein kinases cAMP-dependent protein kinase, protein kinase Cepsilon, and RAF-1 regulates amphotropic murine leukemia virus envelope protein-induced syncytium formation. J Biol Chem 280:16772-83

Feldman, Steven A; Farrell, Karen B; Murthy, Ravi K et al. (2004) Identification of an extracellular domain within the human PiT2 receptor that is required for amphotropic murine leukemia virus binding. J Virol 78:595-602

Khadeer, Mohammed A; Tang, Zhihui; Tenenhouse, Harriet S et al. (2003) Na+-dependent phosphate transporters in the murine osteoclast: cellular distribution and protein interactions. Am J Physiol Cell Physiol 284:C1633-44