Abstract

Studies of the extracellular matrix over the track upon which the LHRH cells migrate from the olfactory placode to the CNS have shown that the appearance of N-CAM directly correlates with the appearance of LHRH cells, whereas fibronectin does not. LHRH cells are present in tissue cultures derived from embryonic nasal regions of mice. A transgenic line was established using a human LHRH promoter joined to the SV40 t-antigen construct. These animals show abnormal reproductive function and on examination very few LHRH immunopositive cells were detected in the forebrain. Transgenic mice containing constructs which have both AVP and OT genes together exhibit cell-specific expression, whereas transgenic mice with only one of these genes exhibit only ectopic expression. Studies of aldose reductase mRNA in lens and kidney show that expression of this gene is inhibited by galactosemia and hyperglycemia. Rat brain slice-explant cultures have been used to show that estrogen and inhibition of spontaneous electrical activity by TTX leads to inhibition of LHRH gene expression. Exaggerated coexistence of CGRP and substance P peptides in postnatal sensory neurons in vitro was found.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Intramural Research (Z01)
Project #
1Z01NS002723-04
Application #
3881774
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
4
Fiscal Year
1990
Total Cost
Indirect Cost

  Institution

Name
National Institute of Neurological Disorders and Stroke
Department
Type
DUNS #
City
State
Country
United States
Zip Code

  Publications

Rusnak, Milan; Toth, Zsuzsanna E; House, Shirley B et al. (2007) Depolarization and neurotransmitter regulation of vasopressin gene expression in the rat suprachiasmatic nucleus in vitro. J Neurosci 27:141-51
Ponzio, Todd A; Yue, Chunmei; Gainer, Harold (2007) An intron-based real-time PCR method for measuring vasopressin gene transcription. J Neurosci Methods 164:149-54
House, Shirley B; Rusnak, Milan; Liu, Xiu-Huai et al. (2006) Bcl-xL and caspase inhibition increase the survival of rat oxytocin and vasopressin magnocellular neurons in organotypic culture. Exp Neurol 200:267-71
Mutsuga, Noriko; Gainer, Harold (2006) Molecular analysis of the magnocellular neuroendocrine phenotype: from the micropunch to laser microdissection. Neurochem Res 31:189-99
Yue, Chunmei; Mutsuga, Noriko; Scordalakes, Elka M et al. (2006) Studies of oxytocin and vasopressin gene expression in the rat hypothalamus using exon- and intron-specific probes. Am J Physiol Regul Integr Comp Physiol 290:R1233-41
Yue, Chunmei; Mutsuga, Noriko; Verbalis, Joseph et al. (2006) Microarray analysis of gene expression in the supraoptic nucleus of normoosmotic and hypoosmotic rats. Cell Mol Neurobiol 26:959-78
Glasgow, Eric; Ryu, Seung-Lim; Yamashita, Mitsuo et al. (2005) APeg3, a novel paternally expressed gene 3 antisense RNA transcript specifically expressed in vasopressinergic magnocellular neurons in the rat supraoptic nucleus. Brain Res Mol Brain Res 137:143-51
Rusnak, Milan; Gainer, Harold (2005) Differential effects of forskolin on tyrosine hydroxylase gene transcription in identified brainstem catecholaminergic neuronal subtypes in organotypic culture. Eur J Neurosci 21:889-98
Zhang, Bing-Jun; Yamashita, Mitsuo; Fields, Ray et al. (2005) EGFP-tagged vasopressin precursor protein sorting into large dense core vesicles and secretion from PC12 cells. Cell Mol Neurobiol 25:581-605
Mutsuga, Noriko; Shahar, Tal; Verbalis, Joseph G et al. (2005) Regulation of gene expression in magnocellular neurons in rat supraoptic nucleus during sustained hypoosmolality. Endocrinology 146:1254-67

Showing the most recent 10 out of 33 publications