The primary objective of this work is to elucidate the cellular and synaptic mechanisms responsible for the genesis of spontaneous rhythmic activity in the developing spinal cord. Experiments are performed on isolated preparations of the chick and mouse spinal cords maintained in vitro. We use electrophysiological, optical and anatomical methods to analyze the function and properties of the developing networks. Recently we have formulated a qualitative model to account for the genesis of rhythmic activity by developing spinal networks. Crucial to this model are our recent observations that network activity and synaptic transmission are depressed after a spontaneous episode. This depression arises in part because of a decrease in the responsiveness of postsynaptic glutamate and GABA receptors in part due to ionic redistribution during an episode. In isolated neonatal mice cords it has been possible to activate locomotor networks using dorsal root stimulation or bath-applied drugs. Once active, this network drives contralateral motoneurons to discharge in an alternating manner. Studies are currently in progress to identify the spinal interneurons responsible for this pattern of activity.

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Intramural Research (Z01)
Project #
1Z01NS002787-13
Application #
6541872
Study Section
(LNLC)
Project Start
Project End
Budget Start
Budget End
Support Year
13
Fiscal Year
2001
Total Cost
Indirect Cost
City
State
Country
United States
Zip Code
Bonnot, Agnes; Chub, Nikolai; Pujala, Avinash et al. (2009) Excitatory actions of ventral root stimulation during network activity generated by the disinhibited neonatal mouse spinal cord. J Neurophysiol 101:2995-3011
Jean-Xavier, Celine; Mentis, George Z; O'Donovan, Michael J et al. (2007) Dual personality of GABA/glycine-mediated depolarizations in immature spinal cord. Proc Natl Acad Sci U S A 104:11477-82
Oz, Murat; Yang, Keun-Hang; Shippenberg, Toni S et al. (2007) Cholecystokinin B-type receptors mediate a G-protein-dependent depolarizing action of sulphated cholecystokinin ocatapeptide (CCK-8s) on rodent neonatal spinal ventral horn neurons. J Neurophysiol 98:1108-14
Personius, Kirkwood E; Chang, Qiang; Mentis, George Z et al. (2007) Reduced gap junctional coupling leads to uncorrelated motor neuron firing and precocious neuromuscular synapse elimination. Proc Natl Acad Sci U S A 104:11808-13
Chub, Nikolai; Mentis, George Z; O'donovan, Michael J (2006) Chloride-sensitive MEQ fluorescence in chick embryo motoneurons following manipulations of chloride and during spontaneous network activity. J Neurophysiol 95:323-30
Mentis, George Z; Siembab, Valerie C; Zerda, Ricardo et al. (2006) Primary afferent synapses on developing and adult Renshaw cells. J Neurosci 26:13297-310
Tabak, Joel; O'Donovan, Michael J; Rinzel, John (2006) Differential control of active and silent phases in relaxation models of neuronal rhythms. J Comput Neurosci 21:307-28
Mentis, George Z; Gravell, Maneth; Hamilton, Rebecca et al. (2006) Transduction of motor neurons and muscle fibers by intramuscular injection of HIV-1-based vectors pseudotyped with select rabies virus glycoproteins. J Neurosci Methods 157:208-17
Oz, Murat; Yang, Keun-Hang; O'donovan, Michael J et al. (2005) Presynaptic angiotensin II AT1 receptors enhance inhibitory and excitatory synaptic neurotransmission to motoneurons and other ventral horn neurons in neonatal rat spinal cord. J Neurophysiol 94:1405-12
O'Donovan, Michael J (2005) Serotonergic neurones drive spontaneous activity in the developing mouse hindbrain. J Physiol 566:643

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