Abstract

LHRH neurons are derived from the olfactory placode and migrate into the brain. The factors which dictate cell-specific LHRH gene expression and the migratory mechanism(s) used by the LHRH neurons during their movement in the CNS are currently unknown. Using in vivo and in vitro models, we are examining the prenatal development of the LHRH system. We are testing the hypothesis that N-CAM and fibronectin direct LHRH movement in nasal regions by using embryonic explants which maintain large numbers of LHRH expressing cells. In addition, we have found that LHRH cells do not posttranslationally modify LHRH to the amidated form in vivo until reaching the forebrain. Embryonic LHRH cells derived from nasal regions were able to produce the """"""""mature"""""""" peptide in vitro in the absence of brain tissue, suggesting that this is a time-dependent (but not spatially-dependent) event.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Intramural Research (Z01)
Project #
1Z01NS002824-02
Application #
3846300
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
2
Fiscal Year
1992
Total Cost
Indirect Cost

  Institution

Name
National Institute of Neurological Disorders and Stroke
Department
Type
DUNS #
City
State
Country
United States
Zip Code

  Publications

Dairaghi, Leigh; Flannery, Ellen; Giacobini, Paolo et al. (2018) Reelin Can Modulate Migration of Olfactory Ensheathing Cells and Gonadotropin Releasing Hormone Neurons via the Canonical Pathway. Front Cell Neurosci 12:228
Turan, Ihsan; Hutchins, B Ian; Hacihamdioglu, Bulent et al. (2017) CCDC141 Mutations in Idiopathic Hypogonadotropic Hypogonadism. J Clin Endocrinol Metab 102:1816-1825
Whittington, Niteace C; Wray, Susan (2017) Suppression of Red Blood Cell Autofluorescence for Immunocytochemistry on Fixed Embryonic Mouse Tissue. Curr Protoc Neurosci 81:2.28.1-2.28.12
Hutchins, B Ian; Kotan, L Damla; Taylor-Burds, Carol et al. (2016) CCDC141 Mutation Identified in Anosmic Hypogonadotropic Hypogonadism (Kallmann Syndrome) Alters GnRH Neuronal Migration. Endocrinology 157:1956-66
Klenke, Ulrike; Taylor-Burds, Carol; Wray, Susan (2014) Metabolic influences on reproduction: adiponectin attenuates GnRH neuronal activity in female mice. Endocrinology 155:1851-63
Kotan, L Damla; Hutchins, B Ian; Ozkan, Yusuf et al. (2014) Mutations in FEZF1 cause Kallmann syndrome. Am J Hum Genet 95:326-31
Constantin, Stephanie; Caligioni, Claudia Simone; Stojilkovic, Stanko et al. (2009) Kisspeptin-10 facilitates a plasma membrane-driven calcium oscillator in gonadotropin-releasing hormone-1 neurons. Endocrinology 150:1400-12
Constantin, Stephanie; Caraty, Alain; Wray, Susan et al. (2009) Development of gonadotropin-releasing hormone-1 secretion in mouse nasal explants. Endocrinology 150:3221-7
Giacobini, Paolo; Wray, Susan (2008) Prenatal expression of cholecystokinin (CCK) in the central nervous system (CNS) of mouse. Neurosci Lett 438:96-101
Constantin, Stephanie; Wray, Susan (2008) Gonadotropin-releasing hormone-1 neuronal activity is independent of hyperpolarization-activated cyclic nucleotide-modulated channels but is sensitive to protein kinase a-dependent phosphorylation. Endocrinology 149:3500-11

Showing the most recent 10 out of 36 publications