Experimental Stroke Models

Hallenback, J M.

Abstract

In a focal model of brain ischemia in the spontaneously hypertensive rat, microvascular perfusion, accumulation of inflammatory mediators, and indices of cellular injury have been correlated spatially and temporally by means of multiple label immunohistochemistry in order to study their role in acute stroke. The findings clearly demonstrate that progressive impairment of microcirculation occurs in regions of brain that undergo progressive damage in the early hours of ischemia. Depletion of leukocytes with the anti-leukocyte antibody, RP-3, and inhibition of tumor necrosis factor-alpha (TNF-alpha) with TNF-binding protein reduce postischemic brain injury. In a mouse model of focal ischemia, prior exposure to TNF-alpha has been shown to induce ischemic tolerance. Oral tolerance to myelin basic protein has been shown to reduce brain injury after focal ischemia in the Lewis rat, implicating immune and inflammatory mechanisms in stroke.

Funding Agency

• Agency: National Institute of Health (NIH)
• Institute: National Institute of Neurological Disorders and Stroke (NINDS)
• Type: Intramural Research (Z01)
• Project #: 1Z01NS002924-01
• Application #: 2468507
• Study Section: Surgery and Bioengineering Study Section (SB)
• Support Year: 1
• Fiscal Year: 1996

Institution

• Name: National Institute of Neurological Disorders and Stroke
• Country: United States

Publications

Mou, Yongshan; Wilgenburg, Brian J; Lee, Yang-ja et al. (2013) A method for hypothermia-induction and maintenance allows precise body and brain temperature control in mice. J Neurosci Methods 213:1-5

Batra, Ayush; Latour, Lawrence L; Ruetzler, Christl A et al. (2010) Increased plasma and tissue MMP levels are associated with BCSFB and BBB disruption evident on post-contrast FLAIR after experimental stroke. J Cereb Blood Flow Metab 30:1188-99

Nakayama, Toshiyuki; Illoh, Kachikwu; Ruetzler, Christl et al. (2007) Intranasal administration of E-selectin to induce immunological tolerization can suppress subarachnoid hemorrhage-induced vasospasm implicating immune and inflammatory mechanisms in its genesis. Brain Res 1132:177-84

Hallenbeck, John (2007) Adaptive Immunity: Introduction. Stroke 38:768-769

Chamorro, Angel; Hallenbeck, John (2006) The harms and benefits of inflammatory and immune responses in vascular disease. Stroke 37:291-3

Illoh, Kachi; Campbell, Catherine; Illoh, Orieji et al. (2006) Mucosal tolerance to E-selectin and response to systemic inflammation. J Cereb Blood Flow Metab 26:1538-50

Chen, Yun; Samal, Babru; Hamelink, Carol R et al. (2006) Neuroprotection by endogenous and exogenous PACAP following stroke. Regul Pept 137:4-19

Hallenbeck, John; Del Zoppo, Gregory; Jacobs, Tom et al. (2006) Immunomodulation strategies for preventing vascular disease of the brain and heart: workshop summary. Stroke 37:3035-42

Hallenbeck, John M; Hansson, Goran K; Becker, Kyra J (2005) Immunology of ischemic vascular disease: plaque to attack. Trends Immunol 26:550-6

Hillion, Joelle A; Takahashi, Kenzo; Maric, Dragan et al. (2005) Development of an ischemic tolerance model in a PC12 cell line. J Cereb Blood Flow Metab 25:154-62