My laboratory has developed and applied molecular cytogenetic techniques, namely comparative genomic hybridization (CGH) and spectral karyotyping (SKY) to identify recurring chromosomal aberrations in solid tumors and hematological malignancies. CGH is a screening test for chromosomal imbalances in tumor genomes (Kallioniemi et al., 1992), and was used to analyze sequential changes in solid tumor progression after tissue microdissection of histologically defined lesions during the genesis of solid tumors. SKY allows color karyotyping of human chromosomes, therefore greatly facilitating the analysis of complex chromosomal aberrations in cancer cells (Schrock et al., 1996). SKY was applied to delineate the structural organization of chromosomal aberrations and to identify novel recurring translocations in carcinomas, sarcomas, and hematological malignancies. We were particularly interested whether:* we can identify a recurring and tumor specific pattern of chromosomal gains and losses* SKY analysis of hematological malignancies can contribute to a genetic classification of leukemias* balanced, reciprocal chromosomal translocations are significant for the genesis of solid tumors of epithelial origin * we can correlate patterns of chromosomal aberrations with tumor stage, tumor phenotype, additional pertinent genetic parameters, and the clinical course* this pattern of recurring and stage specific chromosomal aberrations bears significance in terms of diagnosis, differential diagnosis and prognostication of solid tumors when translated to cytological specimens using interphase cytogenetics - cancer, chromosome, - Neither Human Subjects nor Human Tissues

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Intramural Research (Z01)
Project #
1Z01SC010298-01
Application #
6290866
Study Section
Special Emphasis Panel (MB)
Project Start
Project End
Budget Start
Budget End
Support Year
1
Fiscal Year
1999
Total Cost
Indirect Cost
Name
National Cancer Institute Division of Clinical Sciences
Department
Type
DUNS #
City
State
Country
United States
Zip Code
Rao, V Koneti; Knutsen, Turid; Ried, Thomas et al. (2005) The extent of chromosomal aberrations induced by chemotherapy in non-human primates depends on the schedule of administration. Mutat Res 583:105-19
McNeil, Nicole; Kim, Joong Su; Ried, Thomas et al. (2005) Extraosseous IL-6 transgenic mouse plasmacytoma sometimes lacks Myc-activating chromosomal translocation. Genes Chromosomes Cancer 43:137-46
Park, Sung Sup; Kim, Joong Su; Tessarollo, Lino et al. (2005) Insertion of c-Myc into Igh induces B-cell and plasma-cell neoplasms in mice. Cancer Res 65:1306-15
Han, Seong Su; Shaffer, Arthur L; Peng, Liangping et al. (2005) Molecular and cytological features of the mouse B-cell lymphoma line iMycEmu-1. Mol Cancer 4:40
Barenboim-Stapleton, Linda; Yang, Xuezhong; Tsokos, Maria et al. (2005) Pediatric pancreatoblastoma: histopathologic and cytogenetic characterization of tumor and derived cell line. Cancer Genet Cytogenet 157:109-17
Bowen, T J; Yakushiji, Hiroyuki; Montagna, Cristina et al. (2005) Atm heterozygosity cooperates with loss of Brca1 to increase the severity of mammary gland cancer and reduce ductal branching. Cancer Res 65:8736-46
Knutsen, Turid; Gobu, Vasuki; Knaus, Rodger et al. (2005) The interactive online SKY/M-FISH & CGH database and the Entrez cancer chromosomes search database: linkage of chromosomal aberrations with the genome sequence. Genes Chromosomes Cancer 44:52-64
Schaeffer, Anthony J; Nguyen, Marie; Liem, Amy et al. (2004) E6 and E7 oncoproteins induce distinct patterns of chromosomal aneuploidy in skin tumors from transgenic mice. Cancer Res 64:538-46
Musio, Antonio; Mariani, Tullio; Montagna, Cristina et al. (2004) Recapitulation of the Roberts syndrome cellular phenotype by inhibition of INCENP, ZWINT-1 and ZW10 genes. Gene 331:33-40
Lucas, Philip J; McNeil, Nicole; Hilgenfeld, Eva et al. (2004) Transforming growth factor-beta pathway serves as a primary tumor suppressor in CD8+ T cell tumorigenesis. Cancer Res 64:6524-9

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