Abstract

Epstein Barr virus (EBV) is associated with 200,000 cases of cancer each year worldwide and is the cause of infectious mononucleosis. The virus is also associated with autoimmune diseases including multiple sclerosis. This year we developed a rapid assay to measure EBV antibodies in human sera. The assay involves incubation of sera with an EBV EBNA-luciferase fusion protein, adding paramagnetic protein A/G beads, collecting the beads with the immune complexes, adding luciferase substrate and measuring the light produced with a luminometer. The 45 second assay has 92% sensitivity and 100% specificity with virtually the same diagnostic performance as the FDA-approved commercial EBV VCA ELISA which takes several hours. The assay is also highly quantitative and requires 0.1 ul of sera to perform. This assay could be used as a point-of-care test for diagnosis of EBV infection or to identify seronegative volunteers for vaccine studies.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Investigator-Initiated Intramural Research Projects (ZIA)
Project #
1ZIAAI000058-44
Application #
9786276
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
44
Fiscal Year
2018
Total Cost
Indirect Cost

  Institution

Name
Niaid Extramural Activities
Department
Type
DUNS #
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State
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  Publications

Cohen, Jeffrey I (2018) Herpesviruses in the Activated Phosphatidylinositol-3-Kinase-? Syndrome. Front Immunol 9:237
Coghill, Anna E; Bu, Wei; Hsu, Wan-Lun et al. (2018) Evaluation of Total and IgA-Specific Antibody Targeting Epstein-Barr Virus Glycoprotein 350 and Nasopharyngeal Carcinoma Risk. J Infect Dis 218:886-891
Cohen, Jeffrey I (2018) Author Correction: New activities for old antibiotics. Nat Microbiol 3:844
Cohen, Jeffrey I (2018) New activities for old antibiotics. Nat Microbiol 3:531-532
Bollard, Catherine M; Cohen, Jeffrey I (2018) How I treat T-cell chronic active Epstein-Barr virus disease. Blood 131:2899-2905
Sadaoka, Tomohiko; Schwartz, Cindi L; Rajbhandari, Labchan et al. (2018) Human Embryonic Stem Cell-Derived Neurons Are Highly Permissive for Varicella-Zoster Virus Lytic Infection. J Virol 92:
Burbelo, Peter D; Gunti, Sreenivasulu; Keller, Jason M et al. (2017) Ultrarapid Measurement of Diagnostic Antibodies by Magnetic Capture of Immune Complexes. Sci Rep 7:3818
Cohen, Jeffrey I (2017) GATA2 Deficiency and Epstein-Barr Virus Disease. Front Immunol 8:1869
Wang, Kening; Hoshino, Yo; Dowdell, Kennichi et al. (2017) Glutamine supplementation suppresses herpes simplex virus reactivation. J Clin Invest 127:2626-2630
Liu, XueQiao; Cohen, Jeffrey I (2016) Epstein-Barr Virus (EBV) Tegument Protein BGLF2 Promotes EBV Reactivation through Activation of the p38 Mitogen-Activated Protein Kinase. J Virol 90:1129-38

Showing the most recent 10 out of 32 publications