Abstract

In FY2018, we have continued to explore each stage of the HPV infectious cycle. We have used quasivirus HPV infection of keratinocytes to study early events in HPV genome infection and establishment. We have defined the cis elements required for persistent HPV replication and, in collaboration with others, have conducted in situ HiC chromosome capture analysis to determine the regions where HPV genomes tether to host chromosomes. We are studying the late stage of HPV replication using high resolution microscopy to define the functional architecture of HPV replication foci and are developing cell lines to visualize the formation of these foci in real time. We have also shown that the cellular protein, Sp100 colocalizes with HPV Replication Foci and restricts the productive stage of the infectious cycle.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Investigator-Initiated Intramural Research Projects (ZIA)
Project #
1ZIAAI000713-25
Application #
9786321
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
25
Fiscal Year
2018
Total Cost
Indirect Cost

  Institution

Name
Niaid Extramural Activities
Department
Type
DUNS #
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State
Country
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  Publications

McBride, Alison A; Münger, Karl (2018) Expert Views on HPV Infection. Viruses 10:
Bentley, Peris; Tan, Min Jie Alvin; McBride, Alison A et al. (2018) The SMC5/6 complex interacts with the papillomavirus E2 protein and influences maintenance of viral episomal DNA. J Virol :
McBride, Alison A (2017) Oncogenic human papillomaviruses. Philos Trans R Soc Lond B Biol Sci 372:
Stepp, Wesley H; Stamos, James D; Khurana, Simran et al. (2017) Sp100 colocalizes with HPV replication foci and restricts the productive stage of the infectious cycle. PLoS Pathog 13:e1006660
Moquin, Stephanie A; Thomas, Sean; Whalen, Sean et al. (2017) The Epstein-Barr virus episome maneuvers between nuclear chromatin compartments during reactivation. J Virol :
McBride, Alison A (2017) The Promise of Proteomics in the Study of Oncogenic Viruses. Mol Cell Proteomics 16:S65-S74
Porter, Samuel S; Stepp, Wesley H; Stamos, James D et al. (2017) Host cell restriction factors that limit transcription and replication of human papillomavirus. Virus Res 231:10-20
McBride, Alison A (2017) Mechanisms and strategies of papillomavirus replication. Biol Chem 398:919-927
Van Doorslaer, Koenraad; Chen, Dan; Chapman, Sandra et al. (2017) Persistence of an Oncogenic Papillomavirus Genome Requires cis Elements from the Viral Transcriptional Enhancer. MBio 8:
Van Doorslaer, Koenraad; McBride, Alison A (2016) Molecular archeological evidence in support of the repeated loss of a papillomavirus gene. Sci Rep 6:33028

Showing the most recent 10 out of 25 publications