The group began in April 2009 and the projects were assigned in July 2009. We are organizing the laboratory, hiring personnel and designing experiments. The equipment and reagents are being purchased and the laboratory space adjusted for housing the mass spectrometers. We have purchased the LTQ-Orbitrap and TSQ Quantum Vantage from Thermo Fisher, and the nano-LC systems. These instruments should cover the range of initially planned experiments. We are designing the methods for absolute quantification, developing and testing standards of tagged proteins (in collaboration with Dr. Iain Fraser) and designing peptides for AQUA method of MS-based quantification.
| Manes, Nathan P; Nita-Lazar, Aleksandra (2018) Application of targeted mass spectrometry in bottom-up proteomics for systems biology research. J Proteomics 189:75-90 |
| Manes, Nathan P; Nita-Lazar, Aleksandra (2017) The development of SRM assays is transforming proteomics research. Proteomics 17: |
| Manes, Nathan P; Mann, Jessica M; Nita-Lazar, Aleksandra (2015) Selected Reaction Monitoring Mass Spectrometry for Absolute Protein Quantification. J Vis Exp :e52959 |
| Manes, Nathan P; Angermann, Bastian R; Koppenol-Raab, Marijke et al. (2015) Targeted Proteomics-Driven Computational Modeling of Macrophage S1P Chemosensing. Mol Cell Proteomics : |
| An, Eunkyung; Narayanan, Manikandan; Manes, Nathan P et al. (2014) Characterization of functional reprogramming during osteoclast development using quantitative proteomics and mRNA profiling. Mol Cell Proteomics 13:2687-704 |
| Bui, Minh; Dimitriadis, Emilios K; Hoischen, Christian et al. (2012) Cell-cycle-dependent structural transitions in the human CENP-A nucleosome in vivo. Cell 150:317-26 |
| Rana, Nadia A; Nita-Lazar, Aleksandra; Takeuchi, Hideyuki et al. (2011) O-glucose trisaccharide is present at high but variable stoichiometry at multiple sites on mouse Notch1. J Biol Chem 286:31623-37 |
| Takeuchi, Hideyuki; Fernandez-Valdivia, Rodrigo C; Caswell, Devin S et al. (2011) Rumi functions as both a protein O-glucosyltransferase and a protein O-xylosyltransferase. Proc Natl Acad Sci U S A 108:16600-5 |
| Germain, Ronald N; Meier-Schellersheim, Martin; Nita-Lazar, Aleksandra et al. (2011) Systems biology in immunology: a computational modeling perspective. Annu Rev Immunol 29:527-85 |
