Abstract

Utilization of a very small anomalous diffraction signal provided by sulfur atoms of the cysteine and methionine residues, intrinsically present in all proteins can be succssfully used to solve crystal structures of proteins. This approach, in part pioneered by us, is currently gaining increasoing popularity and may become a routine technique in the practice of macromolecular crystallography. The diffraction data to extremely high resolution of 0.65 angstrom were measured using synchrotron radiation, and the refined model reveals the unprecedented structural details of the investigated molecules. including bonding electrons. The observed very fine fatures in this structure will serve to improve the stereochemical libraries used for routine refinement of protein crystal structures at lower resolution. We participated in several collaborative structural projects on various biologically important proteins, such as the studies of the cold-adapted esterase and DNA-binding protein Taz2.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Investigator-Initiated Intramural Research Projects (ZIA)
Project #
1ZIABC010378-11
Application #
8157288
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
11
Fiscal Year
2010
Total Cost
$1,404,397
Indirect Cost

  Institution

Name
National Cancer Institute Division of Basic Sciences
Department
Type
DUNS #
City
State
Country
Zip Code

  Publications

Luo, Zhipu; Dauter, Zbigniew (2018) The crystal structure of Z-DNA with untypically coordinated Ca2+ ions. J Biol Inorg Chem 23:253-259
Wlodawer, Alexander; Dauter, Zbigniew; Porebski, Przemyslaw J et al. (2018) Detect, correct, retract: How to manage incorrect structural models. FEBS J 285:444-466
Dauter, Zbigniew; Jaskolski, Mariusz (2018) On the helical arrangements of protein molecules. Protein Sci 27:643-652
Dauter, Zbigniew; Wlodawer, Alexander (2018) Unexpected Specificity of a Trypsin-like Enzyme. Structure 26:530-531
Sekula, Bartosz; Ruszkowski, Milosz; Dauter, Zbigniew (2018) Structural Analysis of Phosphoserine Aminotransferase (Isoform 1) From Arabidopsis thaliana- the Enzyme Involved in the Phosphorylated Pathway of Serine Biosynthesis. Front Plant Sci 9:876
Sekula, Bartosz; Dauter, Zbigniew (2018) Crystal structure of thermospermine synthase from Medicago truncatula and substrate discriminatory features of plant aminopropyltransferases. Biochem J 475:787-802
Ruszkowski, Milosz; Sekula, Bartosz; Ruszkowska, Agnieszka et al. (2018) Chloroplastic Serine Hydroxymethyltransferase From Medicago truncatula: A Structural Characterization. Front Plant Sci 9:584
Pyra, Anna; Brzozowska, Ewa; Pawlik, Krzysztof et al. (2017) Tail tubular protein A: a dual-function tail protein of Klebsiella pneumoniae bacteriophage KP32. Sci Rep 7:2223
Luo, Zhipu; Dauter, Zbigniew (2017) Embarras de richesses - It is not good to be too anomalous: Accurate structure of selenourea, a chiral crystal of planar molecules. PLoS One 12:e0171740
Dauter, Miroslawa; Dauter, Zbigniew (2017) Many Ways to Derivatize Macromolecules and Their Crystals for Phasing. Methods Mol Biol 1607:349-356

Showing the most recent 10 out of 96 publications