Utilization of a very small anomalous diffraction signal provided by sulfur atoms of the cysteine and methionine residues or by phosphorus atoms of nucleic acids, intrinsically present in all proteins or RNA/DNA can be successfully used to solve crystal structures of proteins. This approach, in part pioneered by us, is currently gaining increasing popularity and becomes a routine technique in the practice of macromolecular crystallography. The diffraction data to extremely high resolution in the range of 0.55-0.7 angstrom were measured using synchrotron radiation from crystals of Z-DNA and oligopeptides, and the refined model reveals the unprecedented structural details of the investigated molecules. including bonding electrons. The observed very fine features in this structures will serve to improve the stereochemical libraries used for routine refinement of protein crystal structures at lower resolution. We participated in several collaborative structural projects on various biologically important proteins, such as the studies of the cold-adapted esterase and DNA-binding protein Taz2.

National Institute of Health (NIH)
National Cancer Institute (NCI)
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National Cancer Institute Division of Basic Sciences
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Luo, Zhipu; Dauter, Zbigniew (2018) The crystal structure of Z-DNA with untypically coordinated Ca2+ ions. J Biol Inorg Chem 23:253-259
Wlodawer, Alexander; Dauter, Zbigniew; Porebski, Przemyslaw J et al. (2018) Detect, correct, retract: How to manage incorrect structural models. FEBS J 285:444-466
Dauter, Zbigniew; Jaskolski, Mariusz (2018) On the helical arrangements of protein molecules. Protein Sci 27:643-652
Dauter, Zbigniew; Wlodawer, Alexander (2018) Unexpected Specificity of a Trypsin-like Enzyme. Structure 26:530-531
Sekula, Bartosz; Ruszkowski, Milosz; Dauter, Zbigniew (2018) Structural Analysis of Phosphoserine Aminotransferase (Isoform 1) From Arabidopsis thaliana- the Enzyme Involved in the Phosphorylated Pathway of Serine Biosynthesis. Front Plant Sci 9:876
Sekula, Bartosz; Dauter, Zbigniew (2018) Crystal structure of thermospermine synthase from Medicago truncatula and substrate discriminatory features of plant aminopropyltransferases. Biochem J 475:787-802
Ruszkowski, Milosz; Sekula, Bartosz; Ruszkowska, Agnieszka et al. (2018) Chloroplastic Serine Hydroxymethyltransferase From Medicago truncatula: A Structural Characterization. Front Plant Sci 9:584
Dauter, Zbigniew (2017) Collection of X-Ray Diffraction Data from Macromolecular Crystals. Methods Mol Biol 1607:165-184
Luo, Zhipu; Dauter, Zbigniew; Gilski, Miroslaw (2017) Four highly pseudosymmetric and/or twinned structures of d(CGCGCG)2 extend the repertoire of crystal structures of Z-DNA. Acta Crystallogr D Struct Biol 73:940-951
Pyra, Anna; Brzozowska, Ewa; Pawlik, Krzysztof et al. (2017) Tail tubular protein A: a dual-function tail protein of Klebsiella pneumoniae bacteriophage KP32. Sci Rep 7:2223

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