Abstract

The purpose of the current work is to understand the signaling function, biology, and physiology of the evolutionarily-conserved Gbeta5/R7-RGS/R7BP complex in humans. To this end we are first trying to identify and characterize the functions of the Gbeta5 complex and its components in model systems such as cultured neuronal and/or neuroendocrine cells and cell lines, primary rodent brain neurons, and genetically-modified mice. The effect of the loss-of-function of Gbeta5 and other Gbeta5/R7-RGS/R7BP complex components in knockout mice is currently being examined using developmental, behavioral, immunohistochemical, and neuroanatomical analyses.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Investigator-Initiated Intramural Research Projects (ZIA)
Project #
1ZIADK043304-24
Application #
9553235
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
24
Fiscal Year
2017
Total Cost
Indirect Cost

  Institution

Name
U.S. National Inst Diabetes/Digst/Kidney
Department
Type
DUNS #
City
State
Country
Zip Code

  Publications

Pandey, Mritunjay; Zhang, Jian-Hua; Mishra, Santosh K et al. (2017) A central role for R7bp in the regulation of itch sensation. Pain 158:931-944
Lodder, Elisabeth M; De Nittis, Pasquelena; Koopman, Charlotte D et al. (2016) GNB5 Mutations Cause an Autosomal-Recessive Multisystem Syndrome with Sinus Bradycardia and Cognitive Disability. Am J Hum Genet 99:786
Zhang, Jian-Hua; Adikaram, Poorni; Pandey, Mritunjay et al. (2016) Optimization of genome editing through CRISPR-Cas9 engineering. Bioengineered 7:166-74
Nini, Lylia; Zhang, Jian-Hua; Pandey, Mritunjay et al. (2012) Expression of the G?5/R7-RGS protein complex in pituitary and pancreatic islet cells. Endocrine 42:214-7
Zhang, Jian-Hua; Pandey, Mritunjay; Seigneur, Erica M et al. (2011) Knockout of G protein ?5 impairs brain development and causes multiple neurologic abnormalities in mice. J Neurochem 119:544-54
Panicker, Leelamma M; Zhang, Jian-Hua; Posokhova, Ekaterina et al. (2010) Nuclear localization of the G protein beta 5/R7-regulator of G protein signaling protein complex is dependent on R7 binding protein. J Neurochem 113:1101-12