1) We are continuing our work on exosomes as a source of biomarkers, including proteins and miRNA. 2) We are continuing a public-private consortium with the HESI Genomics microRNA Focus Group to compare standard protocol vs site-specific protocols from multiple sites;variations will identify which preanalytical steps affect microRNA detection and quantification in biofluids in drug-induced injury models. The initial model is a cardiotoxin that stimulates release of miRNAs into the circulation, which will shed light on renal handling of miRNA. Urinary miRNA from non-renal sources is expected to be non-exosomal. 3) We continue to work on alternatives to serum creatinine as surrogates for glomuerular filtration rate, such as serum cystatin C.

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Wolfinger, Russell D; Beedanagari, Sudheer; Boitier, Eric et al. (2018) Two approaches for estimating the lower limit of quantitation (LLOQ) of microRNA levels assayed as exploratory biomarkers by RT-qPCR. BMC Biotechnol 18:6
Cai, Yan; Xu, Ming-Jiang; Koritzinsky, Erik H et al. (2017) Mitochondrial DNA-enriched microparticles promote acute-on-chronic alcoholic neutrophilia and hepatotoxicity. JCI Insight 2:
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Bavendam, Tamara G; Norton, Jenna M; Kirkali, Ziya et al. (2016) Advancing a Comprehensive Approach to the Study of Lower Urinary Tract Symptoms. J Urol 196:1342-1349
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Souza, Ana Carolina P; Bocharov, Alexander V; Baranova, Irina N et al. (2016) Antagonism of scavenger receptor CD36 by 5A peptide prevents chronic kidney disease progression in mice independent of blood pressure regulation. Kidney Int 89:809-22
Thompson, Karol L; Boitier, Eric; Chen, Tao et al. (2016) Absolute Measurement of Cardiac Injury-Induced microRNAs in Biofluids across Multiple Test Sites. Toxicol Sci 154:115-125

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