The primary focus of the lab is to develop tools for studying gene function on a genome wide scale. We have recently shifted our mutagenesis efforts from random retroviral integration mutagenesis to using the bacterial CRISPR/Cas9 gene-targeting technique. This provides us with a cost effective way to efficiently target gene KO's in zebrafish. In the past year we have developed a streamlined pipeline for gene targeting using CRISPR/Cas9 where we can use fluorescent PCR and the ABI capillary sequencer to rapidly identify insertions or deletions caused by CRISPR/Cas9 induced double stranded breaks. Using this pipeline we have already targeted over 300 genomic locations and identified the mutations transmitted through the germline. We will continue to develop high-throughput techniques to generate approximately 2,000 targeted gene KO's in the next 3 years. These mutations will feed into other ongoing projects in the lab.
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| Tseng, Wei-Chia; Loeb, Hannah E; Pei, Wuhong et al. (2018) Modeling Niemann-Pick disease type C1 in zebrafish: a robust platform for in vivo screening of candidate therapeutic compounds. Dis Model Mech 11: |
| Pei, Wuhong; Xu, Lisha; Huang, Sunny C et al. (2018) Guided genetic screen to identify genes essential in the regeneration of hair cells and other tissues. NPJ Regen Med 3:11 |
| Watkins-Chow, Dawn E; Varshney, Gaurav K; Garrett, Lisa J et al. (2017) Highly Efficient Cpf1-Mediated Gene Targeting in Mice Following High Concentration Pronuclear Injection. G3 (Bethesda) 7:719-722 |
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| Li, Huiqing; Pei, Wuhong; Vergarajauregui, Sivia et al. (2017) Novel degenerative and developmental defects in a zebrafish model of mucolipidosis type IV. Hum Mol Genet 26:2701-2718 |
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| Burgess, Shawn; Cheng, Linzhao; Gu, Feng et al. (2016) Questions about NgAgo. Protein Cell 7:913-915 |
| Planchart, Antonio; Mattingly, Carolyn J; Allen, David et al. (2016) Advancing toxicology research using in vivo high throughput toxicology with small fish models. ALTEX 33:435-452 |
| Burgess, Shawn (2016) Genomics: A matched set of frog sequences. Nature 538:320-321 |
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