The Facility operating expenses primarily include the cost of annual instrument specific service contracts, covering two laser scanning confocal microscopes and laser microdissection microscope, and purchase of necessary equipment and software upgrades. For example, fluorophore specific fluorescence filter cubes were purchased to optimize imaging of the newly emerging genetically encoded fluorescent proteins. As new technology becomes available, novel imaging procedures are developed and continually refined to take advantage of the full capabilities of the instruments. Image processing and analysis are developed to quantify and standardize observations made using the imaging based assays. Purchasing of reagents, culture dishes, etc. are generally the responsibility of the specific user, but are borne by the facility when directly related to Dr. Kruhlak's work to develop microscope based assays. The Facility provides start up consumable reagents that are replenished by the user.

National Institute of Health (NIH)
National Cancer Institute (NCI)
Scientific Cores Intramural Research (ZIC)
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National Cancer Institute Division of Basic Sciences
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Vizcardo, Raul; Klemen, Nicholas D; Islam, S M Rafiqul et al. (2018) Generation of Tumor Antigen-Specific iPSC-Derived Thymic Emigrants Using a 3D Thymic Culture System. Cell Rep 22:3175-3190
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Kwong, Brandon; Rua, Rejane; Gao, Yuanyuan et al. (2017) T-bet-dependent NKp46+ innate lymphoid cells regulate the onset of TH17-induced neuroinflammation. Nat Immunol 18:1117-1127
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Strickfaden, Hilmar; McDonald, Darin; Kruhlak, Michael J et al. (2016) Poly(ADP-ribosyl)ation-dependent Transient Chromatin Decondensation and Histone Displacement following Laser Microirradiation. J Biol Chem 291:1789-802
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