Abstract

The Facility operating expenses primarily include the cost of annual instrument specific service contracts, covering two laser scanning confocal microscopes and a widefield epifluorescence microscope, and purchase of necessary equipment and software upgrades.As new technology becomes available, novel imaging procedures are developed and continually refined to take advantage of the full capabilities of the instruments. Image processing and analysis are developed to quantify and standardize observations made using the imaging based assays. Purchasing of reagents, culture dishes, etc. are generally the responsibility of the specific user, but are borne by the facility when directly related to Dr. Kruhlak's work to develop microscope based assays. The Facility provides start up consumable reagents that are replenished by the user.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Scientific Cores Intramural Research (ZIC)
Project #
1ZICBC010915-10
Application #
9556805
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
10
Fiscal Year
2017
Total Cost
Indirect Cost

  Institution

Name
Basic Sciences
Department
Type
DUNS #
City
State
Country
Zip Code

  Publications

Vizcardo, Raul; Klemen, Nicholas D; Islam, S M Rafiqul et al. (2018) Generation of Tumor Antigen-Specific iPSC-Derived Thymic Emigrants Using a 3D Thymic Culture System. Cell Rep 22:3175-3190
Klebanoff, Christopher A; Crompton, Joseph G; Leonardi, Anthony J et al. (2017) Inhibition of AKT signaling uncouples T cell differentiation from expansion for receptor-engineered adoptive immunotherapy. JCI Insight 2:
Sharma, Nishi R; Majerciak, Vladimir; Kruhlak, Michael J et al. (2017) KSHV inhibits stress granule formation by viral ORF57 blocking PKR activation. PLoS Pathog 13:e1006677
Kwong, Brandon; Rua, Rejane; Gao, Yuanyuan et al. (2017) T-bet-dependent NKp46+ innate lymphoid cells regulate the onset of TH17-induced neuroinflammation. Nat Immunol 18:1117-1127
Strickfaden, Hilmar; McDonald, Darin; Kruhlak, Michael J et al. (2016) Poly(ADP-ribosyl)ation-dependent Transient Chromatin Decondensation and Histone Displacement following Laser Microirradiation. J Biol Chem 291:1789-802
Sharma, Nishi R; Wang, Xiaohong; Majerciak, Vladimir et al. (2016) Cell Type- and Tissue Context-dependent Nuclear Distribution of Human Ago2. J Biol Chem 291:2302-9
Bhattacharya, Parna; Dey, Ranadhir; Dagur, Pradeep K et al. (2015) Genetically Modified Live Attenuated Leishmania donovani Parasites Induce Innate Immunity through Classical Activation of Macrophages That Direct the Th1 Response in Mice. Infect Immun 83:3800-15
Kaul, Sunil; Mittal, Sharad K; Feigenbaum, Lionel et al. (2015) Expression of the SNARE protein SNAP-23 is essential for cell survival. PLoS One 10:e0118311
Williams, Joy A; Zhang, Jingjing; Jeon, Hyein et al. (2014) Thymic medullary epithelium and thymocyte self-tolerance require cooperation between CD28-CD80/86 and CD40-CD40L costimulatory pathways. J Immunol 192:630-40
Polato, Federica; Callen, Elsa; Wong, Nancy et al. (2014) CtIP-mediated resection is essential for viability and can operate independently of BRCA1. J Exp Med 211:1027-36

Showing the most recent 10 out of 39 publications