Abstract

The Biophysics Resource (BR) is managed and operated by the Structural Biophysics Laboratory (SBL), CCR, NCI. BR provides expert staff to assist users in the design of experiments and operation of advanced biophysical and biophysico-chemical technologies such as circular dichroism spectroscopy, LC/MS and LC/MS/MS spectrometry(including high-resolution option), steady-state fluorescence spectroscopy, isothermal titration calorimetry, differential scanning calorimetry, and both dynamic and static light scattering for characterization and/or study of biomacromolecules or their complexes. The BR provides much more than simple instrument availability by training in the use of instruments, maintenance and upgrades of instrumentation, and consultation/collaboration on experimental design and sophisticated analyses of biophysical experiments. The BR assists researchers from other Institutes on a time-available basis.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Scientific Cores Intramural Research (ZIC)
Project #
1ZICBC010965-02
Application #
7969990
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
2
Fiscal Year
2009
Total Cost
$446,382
Indirect Cost

  Institution

Name
National Cancer Institute Division of Basic Sciences
Department
Type
DUNS #
City
State
Country
Zip Code

  Publications

Stagno, J R; Liu, Y; Bhandari, Y R et al. (2017) Structures of riboswitch RNA reaction states by mix-and-inject XFEL serial crystallography. Nature 541:242-246
Tripathi, Brajendra K; Grant, Tiera; Qian, Xiaolan et al. (2017) Receptor tyrosine kinase activation of RhoA is mediated by AKT phosphorylation of DLC1. J Cell Biol 216:4255-4270
Tewary, Poonam; de la Rosa, Gonzalo; Sharma, Neeraj et al. (2013) ?-Defensin 2 and 3 promote the uptake of self or CpG DNA, enhance IFN-? production by human plasmacytoid dendritic cells, and promote inflammation. J Immunol 191:865-74
Liu, Shan; Chen, Yinghua; Li, Jess et al. (2012) Promiscuous interactions of gp78 E3 ligase CUE domain with polyubiquitin chains. Structure 20:2138-50
Timofeeva, Olga A; Chasovskikh, Sergey; Lonskaya, Irina et al. (2012) Mechanisms of unphosphorylated STAT3 transcription factor binding to DNA. J Biol Chem 287:14192-200
Tarasov, Sergey G; Gaponenko, Vadim; Howard, O M Zack et al. (2011) Structural plasticity of a transmembrane peptide allows self-assembly into biologically active nanoparticles. Proc Natl Acad Sci U S A 108:9798-803
Tu, Chao; Zhou, Xiaomei; Tarasov, Sergey G et al. (2011) The Era GTPase recognizes the GAUCACCUCC sequence and binds helix 45 near the 3' end of 16S rRNA. Proc Natl Acad Sci U S A 108:10156-61
Stagno, Jason R; Altieri, Amanda S; Bubunenko, Mikhail et al. (2011) Structural basis for RNA recognition by NusB and NusE in the initiation of transcription antitermination. Nucleic Acids Res 39:7803-15
Johannessen, Liv; Remsberg, Jarrett; Gaponenko, Vadim et al. (2011) Peptide structure stabilization by membrane anchoring and its general applicability to the development of potent cell-permeable inhibitors. Chembiochem 12:914-21
Li, Chong; Pazgier, Marzena; Li, Jing et al. (2010) Limitations of peptide retro-inverso isomerization in molecular mimicry. J Biol Chem 285:19572-81

Showing the most recent 10 out of 15 publications