CCR Biophysics Resource Core

Tarasov, Sergey

Abstract

The Biophysics Resource (BR) is managed and operated by the Structural Biophysics Laboratory. BR provides expert staff to assist CCR users in the design of experiments and operation of advanced biophysical chemistry technologies such as circular dichroism spectroscopy, LC/MS and LC/MS/MS spectrometry(including high-resolution option), steady-state fluorescence spectroscopy, isothermal titration calorimetry, differential scanning calorimetry, dynamic and static light scattering, microscale thermophoresis for characterization of biomacromolecules and/or their complexes. The BR provides much more than simple instrument availability by training in the use of instruments, maintenance and upgrades of instrumentation, and consultation/collaboration on experimental design and sophisticated analysis of biophysical experiments. The BR assists researchers from other ICs on a time-available basis. The BR is the only place at CCR providing service-oriented access to such technologies as titration and scanning calorimetry, circular dichroism, microscale thermophoresis. Majority of BR technologies, even mass-spectroscopy, are operated in walk-up mode and experiments are run by users after necessary training. It allows minimization of the time interval for complex technologies between project initialization and final result from days and hours down to minutes, if necessary.

Funding Agency

• Agency: National Institute of Health (NIH)
• Institute: National Cancer Institute (NCI)
• Type: Scientific Cores Intramural Research (ZIC)
• Project #: 1ZICBC010965-07
• Application #: 8938445
• Support Year: 7
• Fiscal Year: 2014

Institution

• Name: Basic Sciences

Publications

Stagno, J R; Liu, Y; Bhandari, Y R et al. (2017) Structures of riboswitch RNA reaction states by mix-and-inject XFEL serial crystallography. Nature 541:242-246

Tripathi, Brajendra K; Grant, Tiera; Qian, Xiaolan et al. (2017) Receptor tyrosine kinase activation of RhoA is mediated by AKT phosphorylation of DLC1. J Cell Biol 216:4255-4270

Tewary, Poonam; de la Rosa, Gonzalo; Sharma, Neeraj et al. (2013) ?-Defensin 2 and 3 promote the uptake of self or CpG DNA, enhance IFN-? production by human plasmacytoid dendritic cells, and promote inflammation. J Immunol 191:865-74

Liu, Shan; Chen, Yinghua; Li, Jess et al. (2012) Promiscuous interactions of gp78 E3 ligase CUE domain with polyubiquitin chains. Structure 20:2138-50

Timofeeva, Olga A; Chasovskikh, Sergey; Lonskaya, Irina et al. (2012) Mechanisms of unphosphorylated STAT3 transcription factor binding to DNA. J Biol Chem 287:14192-200

Tarasov, Sergey G; Gaponenko, Vadim; Howard, O M Zack et al. (2011) Structural plasticity of a transmembrane peptide allows self-assembly into biologically active nanoparticles. Proc Natl Acad Sci U S A 108:9798-803

Tu, Chao; Zhou, Xiaomei; Tarasov, Sergey G et al. (2011) The Era GTPase recognizes the GAUCACCUCC sequence and binds helix 45 near the 3' end of 16S rRNA. Proc Natl Acad Sci U S A 108:10156-61

Stagno, Jason R; Altieri, Amanda S; Bubunenko, Mikhail et al. (2011) Structural basis for RNA recognition by NusB and NusE in the initiation of transcription antitermination. Nucleic Acids Res 39:7803-15

Johannessen, Liv; Remsberg, Jarrett; Gaponenko, Vadim et al. (2011) Peptide structure stabilization by membrane anchoring and its general applicability to the development of potent cell-permeable inhibitors. Chembiochem 12:914-21

Li, Chong; Pazgier, Marzena; Li, Jing et al. (2010) Limitations of peptide retro-inverso isomerization in molecular mimicry. J Biol Chem 285:19572-81