The mouse model animal and genotyping core provides support to investigators in the Laboratory of Cancer Biology and Genetics in several areas, all relating to mouse models of B cell lymphoma, myeloma, plasma cell tumorigenesis, skin carcinogenesis, melanoma, breast cancer and prostate cancer, all of which have been developed by various investigators in the group. In the animal core, we maintain the genetic integrity and oversee all of the breeding strategies required to maintain the many different lines of mice. The core staff carries out all genetic monitoring/genotyping. We routinely perform DNA extraction, genotyping of all mouse lines by PCR and gel electrophoresis. We are in the process of outfitting the lab so that we can do Western Blot experiments. We actively participate in the planning, design, execution and analysis of various types of experiments using animal models and in setting up and executing preclinical testing of various drugs for their anti-tumor activity. We have also begun a production breeding program to provide the lab with neonatal mouse pups on a weekly basis for the harvest of keratinocytes. We have acquired an Lumina II imaging machine and can now carry out tumor/drug studies using bioluminescence technology. We write many of the original Animal Study Proposals and associated amendments, and assure that they are kept up to date and in compliance with the NCI Animal Care and Use Committee. We actively participate in preparation for all inspections by the ACUC and AALAC. The animal core is responsible for coordinating the shipment of mouse lines as well as tumor lines to investigators all over the world, thus providing useful research tools to others in the cancer research community. The combination of animal and genomic expertise in the core assists our investigators in their mechanistic studies of cancer initiation, cancer progression and cancer metastasis.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Scientific Cores Intramural Research (ZIC)
Project #
1ZICBC011047-11
Application #
9780230
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
11
Fiscal Year
2018
Total Cost
Indirect Cost
Name
Basic Sciences
Department
Type
DUNS #
City
State
Country
Zip Code
Qian, Jason; Wang, Qiao; Dose, Marei et al. (2014) B cell super-enhancers and regulatory clusters recruit AID tumorigenic activity. Cell 159:1524-37
Zhang, Xinyue; He, Yunlong; Lee, Kyoung-Hwa et al. (2013) Rap2b, a novel p53 target, regulates p53-mediated pro-survival function. Cell Cycle 12:1279-91
Nakahashi, Hirotaka; Kieffer Kwon, Kyong-Rim; Resch, Wolfgang et al. (2013) A genome-wide map of CTCF multivalency redefines the CTCF code. Cell Rep 3:1678-1689
Wu, J Julie; Liu, Jie; Chen, Edmund B et al. (2013) Increased mammalian lifespan and a segmental and tissue-specific slowing of aging after genetic reduction of mTOR expression. Cell Rep 4:913-20
Li, Mangmang; He, Yunlong; Dubois, Wendy et al. (2012) Distinct regulatory mechanisms and functions for p53-activated and p53-repressed DNA damage response genes in embryonic stem cells. Mol Cell 46:30-42
Kovalchuk, Alexander L; Ansarah-Sobrinho, Camilo; Hakim, Ofir et al. (2012) Mouse model of endemic Burkitt translocations reveals the long-range boundaries of Ig-mediated oncogene deregulation. Proc Natl Acad Sci U S A 109:10972-7
LeGrand, Jason; Park, Eun Sung; Wang, Hongyang et al. (2012) Global gene expression profiling in mouse plasma cell tumor precursor and bystander cells reveals potential intervention targets for plasma cell neoplasia. Blood 119:1018-28
Smrz, Daniel; Kim, Mi-Sun; Zhang, Shuling et al. (2011) mTORC1 and mTORC2 differentially regulate homeostasis of neoplastic and non-neoplastic human mast cells. Blood 118:6803-13
Liu, Z; Yang, X; Li, Z et al. (2011) CASZ1, a candidate tumor-suppressor gene, suppresses neuroblastoma tumor growth through reprogramming gene expression. Cell Death Differ 18:1174-83
Zhang, Shuling; Readinger, Julie A; DuBois, Wendy et al. (2011) Constitutive reductions in mTOR alter cell size, immune cell development, and antibody production. Blood 117:1228-38

Showing the most recent 10 out of 13 publications