The Genomics Core Facility is a high quality microarray processing site that can be accessed by any NIDDK P.I. for gene expression analysis. Time permitting, our services are available to other NIH ICs. The Core Staff acquires various sample RNAs from investigators, checks sample quality, makes probes, hybridizes and washes expression arrays, collects data, and deposits all data in a central database that is accessible to DDK staff. Our bioinformatician also provides basic data analysis to all customers to ensure data quality. Additional analysis is provided as requested when feasible. For efficiency and reproducibility we have adopted a single array platform. In the past year, we have expanded our high capacity, parallel sequencing services. Using the Illumina platform, we acquire size-selected samples from researchers, generate flow cell clusters, and capture image/sequence data. That data is then converted to text files of short tag (26-75nt) sequence information and passed back to individual investigators for analysis. We provide limited bioinformatic support for this data at this time with the bulk of the burden for analysis being placed on the individual investigators. This service is open to all DDK P.I.s.

Project Start
Project End
Budget Start
Budget End
Support Year
2
Fiscal Year
2009
Total Cost
$717,781
Indirect Cost
City
State
Country
Zip Code
Krivega, Ivan; Dean, Ann (2018) Chromosome Conformation Capture (3C and Higher) with Erythroid Samples. Methods Mol Biol 1698:237-243
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Zhuang, Lenan; Jang, Younghoon; Park, Young-Kwon et al. (2018) Depletion of Nsd2-mediated histone H3K36 methylation impairs adipose tissue development and function. Nat Commun 9:1796
Krivega, Ivan; Dean, Ann (2018) Chromatin Immunoprecipitation (ChIP) with Erythroid Samples. Methods Mol Biol 1698:229-236
Brown, J Lesley; Sun, Ming-An; Kassis, Judith A (2018) Global changes of H3K27me3 domains and Polycomb group protein distribution in the absence of recruiters Spps or Pho. Proc Natl Acad Sci U S A 115:E1839-E1848

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