Biophysics Core Facility

Piszczek, Grzegorz

Abstract

In the FY2011-12 Biophysics Core Facility served users from 18 NHLBI laboratories, 12 laboratories from other NIH institutes and two collaborators from outside NIH. This work resulted in 4 published papers coauthored by the Facility director and several acknowledgements. List of NHLBI PIs served by the Biophysics Core Facility (by Branch/Center): S. J. Levine, CPB, J. Moss, CPB; A.T. Remaley, CPB; R.M. Kotin, GDBC; P.B. Chock, BBC; A. Ferre-D'Amare, BBC; J.R. Knutson, BBC; R.L. Levine, BBC; J.C. Lee, BBC; K.C. Neuman, BBC; J. Taraska, BBC; N. Tjandra, BBC; J.A. Hammer, CBPC; L.E. Greene, CBPC; R.W. Hendler, CBPC; A. Peterkofsky, CBPC; J.R. Sellers, CBPC; G. Griffiths, IPDC. List of PIs from other NIH Institutes served by the Biophysics Core Facility: M.W. Brechbiel, NCI (Radiation Oncology Branch); S. Subramaniam, NCI (Laboratory of Cell Biology); S. Buchanan, NIDDK (Laboratory of Molecular Biology); K. Gawrisch, NIAAA (Laboratory of Membrane Biochemistry &Biophysics); A. Roll-Mecak, NINDS (Cell Biology and Biophysics Unit); S.P. Becerra, NEI (Laboratory of Retinal Cell and Molecular Biology) Y. Sergeev NEI (Ophthalmic Genetics and Visual Function Branch); W. Wang, NIA (Laboratory of Genetics); T. Xiao, NIAID (Laboratory of Immunology); C.E. Barry, NIAID (Laboratory of Clinical Infectious Diseases); M. Hee Park, NIDCR (Oral and Pharyngeal Cancer Branch); P. Schuck, NIBIB (Dynamics of Macromolecular Assembly Laboratory). Collaborators from outside the NIH: C.A. Brautigam, University of Texas, UT Southwestern Medical Center, Dallas, TX; S. Keller, Molecular Biophysics Laboratory, Faculty of Biology, University of Kaiserslautern, Kaiserslautern (Germany).

Funding Agency

• Agency: National Institute of Health (NIH)
• Institute: National Heart, Lung, and Blood Institute (NHLBI)
• Type: Scientific Cores Intramural Research (ZIC)
• Project #: 1ZICHL000338-12
• Application #: 8558129
• Support Year: 12
• Fiscal Year: 2012
• Total Cost: $342,510

Institution

• Name: National Heart, Lung, and Blood Institute

Publications

Liu, Xiong; Billington, Neil; Shu, Shi et al. (2017) Effect of ATP and regulatory light-chain phosphorylation on the polymerization of mammalian nonmuscle myosin II. Proc Natl Acad Sci U S A 114:E6516-E6525

O'Neil, Patrick K; Richardson, Lynn G L; Paila, Yamuna D et al. (2017) The POTRA domains of Toc75 exhibit chaperone-like function to facilitate import into chloroplasts. Proc Natl Acad Sci U S A 114:E4868-E4876

Hayoz, Sebastien; Tiwari, Purushottam B; Piszczek, Grzegorz et al. (2017) Investigating cyclic nucleotide and cyclic dinucleotide binding to HCN channels by surface plasmon resonance. PLoS One 12:e0185359

Jung, Goeh; Alexander, Christopher J; Wu, Xufeng S et al. (2016) V-1 regulates capping protein activity in vivo. Proc Natl Acad Sci U S A 113:E6610-E6619

Liu, W; Li, H; Hong, S-H et al. (2016) Olfactomedin 4 deletion induces colon adenocarcinoma in Apc(Min/+) mice. Oncogene :

Paez-Segala, Maria G; Sun, Mei G; Shtengel, Gleb et al. (2015) Fixation-resistant photoactivatable fluorescent proteins for CLEM. Nat Methods 12:215-8, 4 p following 218

Zhao, Huaying; Piszczek, Grzegorz; Schuck, Peter (2015) SEDPHAT--a platform for global ITC analysis and global multi-method analysis of molecular interactions. Methods 76:137-148

Zhao, Huaying; Ghirlando, Rodolfo; Alfonso, Carlos et al. (2015) A multilaboratory comparison of calibration accuracy and the performance of external references in analytical ultracentrifugation. PLoS One 10:e0126420

Gruschus, James M; Jiang, Zhiping; Yap, Thai Leong et al. (2015) Dissociation of glucocerebrosidase dimer in solution by its co-factor, saposin C. Biochem Biophys Res Commun 457:561-6

Rout, Ashok K; Strub, Marie-Paule; Piszczek, Grzegorz et al. (2014) Structure of transmembrane domain of lysosome-associated membrane protein type 2a (LAMP-2A) reveals key features for substrate specificity in chaperone-mediated autophagy. J Biol Chem 289:35111-23