The NHLBI Light Microscopy Core Facility (LMF) facility has been in operation for thirteen years. The NHLBI-LMF consists of three people, Dr. Christian A. Combs (Director), Dr. Daniela A. Malide, and Dr. Xufeng Wu (Deputy Director). The facility consists of twenty microscopes located in several locations in buildings 10 and 50 on the main NIH campus in Bethesda, MD. To date we have helped researchers publish more than 190 papers and assisted almost every NHLBI-DIR Center and Branch in the past thirteen years. In 2011-2012 we have help to publish twenty two papers (at the time of this report). Research conducted in the LMF has been on many disease states, basic cell biology, and on development and implementation of new imaging techniques. The general makeup of the NHLBI-LMF reflects the evolving light microscopy needs of NHLBI-DIR researchers and a commitment to provide a maximal level of assistance to the NHLBI mission. The mission of this facility is to provide state-of-the-art equipment, training, and image processing capabilities to assist NHLBI-DIR researchers in experiments involving light microscopy. Researchers that work in this facility can expect support from core personnel to whatever level suits their research. This can include advanced microscopy techniques like intra-vital two-photon microscopy and super-resolution microscopy to more ordinary wide-field imaging with a color camera Our emphasis is on training users to conduct the experiments themselves, although we are available for collaboration and all manner of assistance (experimental planning, data analysis and image processing, etc.) where required. Over the thirteen years this facility has been in existence we have endeavored to provide a flexible and easy to use facility that meets researchers needs and allows them to conduct their experiments in an efficient manner even if they have had no prior microscopy experience. These goals are met in several ways. First, we have an array of microscopes that offer a wide-range of microscopy techniques. These microscopes and the microscopy techniques available have been chosen and developed in response to the specific needs of researchers in the institute. In addition to the microscopes, we provide a full suite of image processing programs in several image processing workstations. Where image-processing capabilities are lacking in these programs we either write our own image processing programs. In summary this facility operates with state of the art equipment and a dedicated team of imaging professionals to further the research mission of the NHLBI-DIR.
| Dong, Fei; Jin, Xueting; Boettler, Michelle A et al. (2018) A Mouse Model of Schnyder Corneal Dystrophy with the N100S Point Mutation. Sci Rep 8:10219 |
| Jin, Xueting; Dimitriadis, Emilios K; Liu, Ying et al. (2018) Macrophages Shed Excess Cholesterol in Unique Extracellular Structures Containing Cholesterol Microdomains. Arterioscler Thromb Vasc Biol 38:1504-1518 |
| Sun, Nuo; Malide, Daniela; Liu, Jie et al. (2017) A fluorescence-based imaging method to measure in vitro and in vivo mitophagy using mt-Keima. Nat Protoc 12:1576-1587 |
| Lucotte, Bertrand M; Powell, Chloe; Knutson, Jay R et al. (2017) Direct visualization of the arterial wall water permeability barrier using CARS microscopy. Proc Natl Acad Sci U S A 114:4805-4810 |
| Glancy, Brian; Hartnell, Lisa M; Combs, Christian A et al. (2017) Power Grid Protection of the Muscle Mitochondrial Reticulum. Cell Rep 19:487-496 |
| Combs, Christian A; Shroff, Hari (2017) Fluorescence Microscopy: A Concise Guide to Current Imaging Methods. Curr Protoc Neurosci 79:2.1.1-2.1.25 |
| Thacker, Seth G; Rousset, Xavier; Esmail, Safiya et al. (2015) Increased plasma cholesterol esterification by LCAT reduces diet-induced atherosclerosis in SR-BI knockout mice. J Lipid Res 56:1282-95 |
| Glancy, Brian; Hartnell, Lisa M; Malide, Daniela et al. (2015) Mitochondrial reticulum for cellular energy distribution in muscle. Nature 523:617-20 |
| Sun, Nuo; Yun, Jeanho; Liu, Jie et al. (2015) Measuring In Vivo Mitophagy. Mol Cell 60:685-96 |
| Rosales, Tilman; Sackett, Dan L; Xu, Jianhua et al. (2015) STAQ: A route toward low power, multicolor nanoscopy. Microsc Res Tech 78:343-55 |
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