Flow Cytometric Analysis of Benign and Malignant Tumors

Stetler-Stevenson, Maryalice

Abstract

The Flow Cytometry Unit provides extensive support for NCI clinical protocols by providing diagnostic testing for leukemia and lymphoma in patients either on NCI clinical protocols or undergoing testing to determine eligibility for said protocols. The Flow Cytometry Unit provides clinical flow cytometric evaluation of blood, bone marrow, fine needle aspirates, tissue biopsies and body fluids for hematolymphoid neoplasia in NCI and NIH patients. The majority of flow cytometric testing performed by the Flow Cytometry Unit involves detection of minimal residual disease (patients are status post therapy at outside institution prior to enrollment on NCI protocol) and measurement of specific tumor cell characteristics mandated by various clinical protocols. Significant effort in the unit is devoted to delivery of numerous unique protocol mandated assays that are not offered by commercial laboratories. The Flow Cytometry Unit is also actively engaged in education of the NIH staff and medical community at large in the proper utility of flow cytometry in medical practice.

Funding Agency

• Agency: National Institute of Health (NIH)
• Institute: National Cancer Institute (NCI)
• Type: Scientific Cores Intramural Research (ZIC)
• Project #: 1ZICSC009372-24
• Application #: 9154363
• Support Year: 24
• Fiscal Year: 2015

Institution

• Name: Clinical Sciences

Publications

Salem, Dalia Abdel-Raouf; Korde, Neha; Venzon, David J et al. (2018) Expression of the IL-6 receptor alpha-chain (CD126) in normal and abnormal plasma cells in monoclonal gammopathy of undetermined significance and smoldering myeloma. Leuk Lymphoma 59:178-186

Herndon, Thomas M; Chen, Shih-Shih; Saba, Nakhle S et al. (2017) Direct in vivo evidence for increased proliferation of CLL cells in lymph nodes compared to bone marrow and peripheral blood. Leukemia 31:1340-1347

Keeney, Michael; Wood, Brent L; Hedley, Benjamin D et al. (2017) A QA program for MRD testing demonstrates that systematic education can reduce discordance among experienced interpreters. Cytometry B Clin Cytom :

Salem, Dalia; Stetler-Stevenson, Maryalice; Yuan, Constance et al. (2016) Myeloma minimal residual disease testing in the United States: Evidence of improved standardization. Am J Hematol 91:E502-E503

Wolniak, Kristy; Goolsby, Charles; Choi, Sarah et al. (2016) Report of the results of the International Clinical Cytometry Society and American Society for Clinical Pathology workload survey of clinical flow cytometry laboratories. Cytometry B Clin Cytom :

Kreitman, Robert J; Stetler-Stevenson, Maryalice; Jaffe, Elaine S et al. (2016) Complete Remissions of Adult T-cell Leukemia with Anti-CD25 Recombinant Immunotoxin LMB-2 and Chemotherapy to Block Immunogenicity. Clin Cancer Res 22:310-8

Rawstron, Andy C; Paiva, Bruno; Stetler-Stevenson, Maryalice (2016) Assessment of minimal residual disease in myeloma and the need for a consensus approach. Cytometry B Clin Cytom 90:21-5

Niemann, Carsten U; Herman, Sarah E M; Maric, Irina et al. (2016) Disruption of in vivo Chronic Lymphocytic Leukemia Tumor-Microenvironment Interactions by Ibrutinib--Findings from an Investigator-Initiated Phase II Study. Clin Cancer Res 22:1572-82

Preffer, Frederic I; Yuan, Constance M; Lin, Pei et al. (2016) Introduction to multiple myeloma special issue: The flow cytometric detection of minimal residual disease. Cytometry B Clin Cytom 90:9-10

Stetler-Stevenson, Maryalice; Paiva, Bruno; Stoolman, Lloyd et al. (2015) Consensus guidelines for myeloma minimal residual disease sample staining and data acquisition. Cytometry B Clin Cytom :