With this award from the Chemistry Research Instrumentation and Facilities: Departmental Multi-User Instrumentation program (CRIF:MU), Professor Arthur Stipanovic and colleagues from SUNY College of Environmental Science and Forestry will acquire a console and probes to upgrade a 600 MHz nuclear magnetic resonance (NMR) spectrometer. The proposal will enhance research training and education at all levels, especially in areas of study such as (a) insect pheromone research, (b) NMR of cell wall polysaccharides, (c) NMR of natural products, (d) NMR utilization in biorefinery applications, (e) subunit structure and interactions in the stator domain of the vacuolar proton-ATPase of eukarotyic cells, and (f) novel biocatalytic structures for the sustainable synthesis of fine chemicals and biologically active substances.
Nuclear Magnetic Resonance (NMR) spectroscopy is one of the most powerful tools available to chemists for the elucidation of the structure of molecules. It is used to identify unknown substances, to follow the progress of chemical reactions, to characterize specific arrangements of atoms within molecules, and to study the dynamics of interactions between molecules in solids and in solution. Access to state-of-the-art NMR spectrometers is essential to carry out frontier chemistry related research and to train students in modern research techniques. The results from these NMR studies will have an impact on organic and bioorganic chemistry research at SUNY-ESF. The instrument will be available to users at other institutions including Syracuse University, SUNY Upstate Medical University and LeMoyne College and for collaborations with regional companies. The resources will be used not only for research activities but also for research training of undergraduate and graduate students including those from underrepresented groups.
This project was funded under the Chemistry Research Instrumentation and Facilities: Departmental Multi-User Instrumentation program (CRIF:MU) to upgrade a 600 MHz NMR instrument that was approximately 12 years old. This instrument is used by researchers in several departments at SUNY-ESF including Chemistry, Environmental Biology, Paper and Bioprocess Engineering and Forestry / Natural Resources Management plus regional (Syracuse area) users at the following institutions: SUNY Upstate Medical University ( Departments of: Biochemistry and Molecular Biology, Pharmacology and the Cancer ResearchInstitute), Syracuse University (Departments of Chemistry, Biology and Civil /Environmental Engineering), LeMoyne College, SUNY Oswego, Cayuga CommunityCollege, Cornell University, and Hobart / William Colleges.The installation of new equipment began in July 2011. This upgraded instrument resulted in the publications listed below: Ryan C. Tappel, Qin Wang, and Christopher T. Nomura, "Precise control of repeating unit composition in biodegradable poly(3-hydroxyalkanoate) polymers synthesized by Escherichia coli", Journal of Bioscience and Bioengineering, VOL. 113 No. 4, 480–486, 2012. doi:10.1016/j.jbiosc.2011.12.004 Christopher. T. Nomura, Qin Wang, Ryan C. Tappel, and Chengjun Zhu, "Development of a New Strategy for Production of Medium-Chain-Length Polyhydroxyalkanoates by Recombinant Escherichia coli via Inexpensive Non-Fatty Acid Feedstocks", Appl. Environ. Microbiol. 78(2):519, 2012. Anna M. Flach, Frederick E. Johnson and Israel Cabasso, "Synthesis and characterization of Fluorinated polyionomers. Part I: polyperfluoro-sulfonylethoxy propylene vinyl ether sulfonimides containing aryl sulfonic acids" Polym. Chem., 4, 3370, (2013) Israel Cabasso, Mingtu Li, and Youxin Yuan" Electrochemical synthesis of a novel compound, 5-acetyl-2,9-decanedione, and theoretical analysis of its lithium ion complex", Royal Soc. Chem. RSC/Advance, 2, 9998–10006 (2012) . Barnhart, D.; Kaplan, D.L.; Winter, W.T.; Panilaitis, B.J.; Kiemle, D.J. (2012) Structural details of the Polyelectrolytic Exopolysaccharide (APE), the stabilizing component of the Acinetobacter venetianus RAG-1 emulsan complex. Carbohydrate Polymers 88 (1): 257-262. Giner, J.-L.; Wang, H.; Morita, C. T.,"Synthesis and immunological evaluation of the 4-beta-glucoside of HMBPP", Bioorg. Med. Chem. Letters 2012, 22, 811-813. Hong, Y. J.; Giner, J.-L.; Tantillo, D. J.,"Triple Shifts and Thioether Assistance in Rearrangements Associated with an Unusual Biomethylation of the Sterol Side Chain", J. Org. Chem. 2013, 78, 935–941. Sallans, L.; Giner, J.-L.; Kiemle, D. J.; Custer, J. E.; Kaneshiro, E. S.,"Structural identities of four glycosylated lipids in the oral bacterium Streptococcus mutans UA159", Biochim. Biophys. Acta 2013, 1831, 1239-1249. Grabenstatter, J.; Méhay, S.; McIntyre-Wressnig, A.; Giner, J.-L.; Edgcomb, V. P.; Beaudoin, D. J.; Bernhard, J. M.; Summons, R. E.,"Identification of 24-n-propylidenecholesterol in a member of the Foraminifera", Org. Geochem. 2013, 63,145-151. Faraldos, J. A.; Coates, R. M.; Giner, J.-L.,"Alternative Synthesis of the Colorado Potato Beetle Pheromone", J. Org. Chem. 2013, 78 - in press. Vaughn, M. M., Wang, Q., Webster, F. X., Kiemle, D., Hong, Y. J., Tantillo, D. J., Coates, R. M., Wray, A. T., Askew, W., O’Donnell, C., Tokuhisa, J. G., Tholl, D. Formation of the unusual semivolatile diterpene rhizathelene by the Arabidopsis class I terpene synthase TPS08 in the root stele is involved in defense against belowground herbivory. Plant Cell, 2013, 1108 – 1125. Kun Cheng, Vincent A. Barber, Mark S. Driscoll, William T. Winter and Arthur J. Stipanovic*, "Reducing Woody Biomass Recalcitrance by Electron Beams, Biodelignification and Hot-water Extraction, Journal of Bioprocess Engineering and the Biorefinery, 2(2), 143-152 (2013). W. Pan, J.A. Perotta, A.J. Stipanovic, C.T. Nomura and J.P. Nakas, "Production of polyhydroxyalkanoates by Burkholderia cepacia ATCC 17759 using a detoxified sugar maple hemicellulosic hydrolysate", Journal of Industrial Microbiology and Biotechnology, 39(3), 1367-5435 (2012). Y. Wang and S. Liu 2013 "Polyhydroxyalkanoates (PHAs): Biosynthesis, Production and Recovery" J. Bioproc. Eng. Biorefinery. 2(1):61-72. J.P. Buyondo and S. Liu 2013 "Unstructured Kinetic Modeling of Batch Production of Lactic Acid from Hemicellulosic Sugars" J. Bioproc. Eng. Biorefinery. 2(1):40-45. Y. Wang, Z. Liu, MAR Chatsko and S. Liu 2013 "Ethanol Fermentation by Escherichia Coli FBWHR Using Hot-Water Sugar Maple Wood Exact Hydrolyzate as Substrate: A Batch Fermentation and Kinetic Study" J. Bioproc. Eng. Biorefinery. 2(1):20-26. J. Yan, N. Joshee and S. Liu 2013 "Kinetics of the Hot-Water Extraction of Paulownia Elongata Woodchips" J. Bioproc. Eng. Biorefinery. 2(1):1-10. Y. Wang, Z. Sun, A. M. Shupe and S. Liu. 2012 "Influence of Oxygen Mass Transfer on the Fermentation Behavior of Burkholderia Cepacia for Polyhydroxyalkanoates (PHAs) Production Utilizing Wood Extract Hydrolysate JWEH)", J. Bioproc. Eng. Biorefinery. 1(2):169-175. A.M. Shupe, D. Kiemle and S. Liu. 2012 "Quantitative 2D HSQC NMR Analysis of Mixed Wood Sugars in Hemicellulosic Hydrolysate Fermentation Broth", J. Bioproc. Eng. Biorefinery. 1(1):93-100. Z. Sun and S. Liu. 2012 "Production of n-Butanol from Concentrated Sugar Maple Hemicellulosic Hydrolysate by Clostridia Acetobutylicum ATCC 824", J. Biomass Bioenergy, 39: 39-47. doi:10.1016/j.biombioe.2010.07.026 Edna S. Kaneshiro, and Larry Sallans, "Structural Identities of Four Glycosylated Lipids in the Oral Bacterium Streptococcus mutans UA159"’ Molecular and Cell Biology of Lipids 1831 (2013), pp. 1239-1249
