This grant from the Organic Dynamics Program supports the continuing work of Professor Paul Dowd at the University of Pittsburgh to study a new experimental model for the vitamin K-dependent carboxylation to further unravel its essential role in the complex events of blood clotting. The oxygenation of vitamin K will be studied using isotopically labeled oxygen. This will be followed by other oxygenations and investigations of the nature of the base(s) involved and of the effects of other strong bases on activity. Intramolecular models, micelles, liposomes and environments that mimic those of the membrane locale of the vitamin K action will also be explored. %%% In this project Dr. Paul Dowd will conduct an extensive set of oxygenation studies, using labeled molecular oxygen, to unravel the complex mechanism of blood clotting in which vitamin K plays an essential role. Not only is a new experimental model used for the action of vitamin K, but also its environment is modelled to mimic the membrane locale where the vitamin is active. The influence of strong bases on vitamin K's activity is an important part of this study.

Agency
National Science Foundation (NSF)
Institute
Division of Chemistry (CHE)
Application #
9302560
Program Officer
Tyrone D. Mitchell
Project Start
Project End
Budget Start
1993-06-01
Budget End
1996-11-30
Support Year
Fiscal Year
1993
Total Cost
$305,000
Indirect Cost
Name
University of Pittsburgh
Department
Type
DUNS #
City
Pittsburgh
State
PA
Country
United States
Zip Code
15213