With funding from the Organic Dynamics Program Professors Warner and Jebaratnam of Northeastern University will synthesize and test a DNA site specific cleavage reagent containing a specific recognition element tethered on both ends with didiazonium ion precursors. Efforts will be made to identify and enhance site specific cleavage of DNA using metal ion catalysis to generate radicals and to study the site specific cleavage caused by the generation of the tethered phenyl cations. This project will undertake the design of unique carbon based compounds which will break large polymer molecules of DNA at selected bonds in the structure into smaller identifiable components. The successful design of this technology could impact studies such as the human genome project, the genesis of genetic diseases, and genetic engineering.

Agency
National Science Foundation (NSF)
Institute
Division of Chemistry (CHE)
Application #
9406469
Program Officer
Tyrone D. Mitchell
Project Start
Project End
Budget Start
1994-06-15
Budget End
1998-05-31
Support Year
Fiscal Year
1994
Total Cost
$295,100
Indirect Cost
Name
Northeastern University
Department
Type
DUNS #
City
Boston
State
MA
Country
United States
Zip Code
02115