Chinese herbal medicines have been widely used in the world. However, the efficacy and mechanisms of these herbal medicines are not well studied. It is imperative to investigate their efficacy using standardized design, and to elucidate the mechanisms of their action using modern technology. By employing an anti-tumor Chinese herbal formula, ZYD88, the current research proposal is designed to test the hypothesis that ZYD88 induces apoptosis in prostate cancer cells in a xenograft animal model with prostate LNCaP tumor. Cell apoptosis will be determined by assaying caspase-3 activation and CK18, the common early apoptotic events, and DNA fragmentation, a hallmark of cell apoptosis in tumor tissue sections. Furthermore, the release of cytochrome c from mitochondria to cytosol and the cellular levels of Bcl-2 family proteins (Bcl-2, BcI-XL, Bax and Bak), events in apoptotic pathway will be determined by Western blotting analysis. The data will be compared between ZYD88 treated and untreated samples. Dose-response and time-course analyses of ZYD88 action will be performed. These studies will facilitate our understanding of the efficacy and mechanisms of Chinese herbal medicine in cancer therapy, a goal of my career. ? ?

Agency
National Institute of Health (NIH)
Institute
National Center for Complementary & Alternative Medicine (NCCAM)
Type
International Research Fellowships (FIC) (F05)
Project #
7F05AT002009-03
Application #
7287969
Study Section
Special Emphasis Panel (ZAT1-CP (14))
Program Officer
Hayes, Deborah
Project Start
2005-03-16
Project End
2008-09-14
Budget Start
2006-09-15
Budget End
2007-09-14
Support Year
3
Fiscal Year
2006
Total Cost
$32,000
Indirect Cost
Name
Institute of Materia Medica
Department
Type
DUNS #
544983760
City
Beijing
State
Country
China
Zip Code
10005-0
Blumer, Roland; Maurer-Gesek, Barbara; Gesslbauer, Bernhard et al. (2016) Palisade Endings Are a Constant Feature in the Extraocular Muscles of Frontal-Eyed, But Not Lateral-Eyed, Animals. Invest Ophthalmol Vis Sci 57:320-31