The calcitonin/CGRP-I (calcitonin gene-related peptide) gene consists of six exons that give rise to two different products by alternative RNA processing: 1)calcitonin produced by C cells of the thyroid and 2) CGRP-I produced in many neuronal cell types. The generation of these involves alternative exon usage. Calcitonin is produced when exon 4 is used with polyadenylation occurring at this exon. cGRP-I is produced by excluding exon 4 and splicing of exon 3 to exon 5 and use of the polyadenylation signal in exon 6. Thus, the difference in 3' splice site choice, either using the 3' splice site at exon 4 or exon 5 generates calcitonin or cGRP-I, respectively. The transacting factor(s) controlling this 3' splice site choice will be identified. The corresponding cDNA will be subcloned into a bacterial expression vector and the protein purified to apparent homogeneity for detailed molecular analysis of this splice site switch.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Postdoctoral Individual National Research Service Award (F32)
Project #
1F32DK009336-01
Application #
2136422
Study Section
General Medicine B Study Section (GMB)
Program Officer
Hyde, James F
Project Start
1996-01-15
Project End
Budget Start
1995-09-01
Budget End
1996-08-31
Support Year
1
Fiscal Year
1995
Total Cost
Indirect Cost
Name
University of Massachusetts Medical School Worcester
Department
Other Basic Sciences
Type
Schools of Medicine
DUNS #
660735098
City
Worcester
State
MA
Country
United States
Zip Code
01655