18. GOALS FOR FELLOWSHIP TRAININGAND CAREER During this fellowship,I would like to studythe fundamental question of how organisms regulate expression of their genes. More specifically,I would like to sudy how transcription of genes is repressed and how this repression is regulated. I would also like to gain some experienceusing bioinformatics techniques to answer biological questions. My long term career goals are to use my previous and current research experiencesto answer the fundamentalbiological questions surrounding gene regulation. I believe it will be critical for the scientist in the futureto be able to do denovo experiments as well as analyze the exponentially accumulating amount of existing data to answer these questions. 19. NAME AND DEGREE(S) Martin Privalsky, B.S., Ph.D. 20. POSITIONRANK Full Professor 21. RESEARCHINTERESTWAREAS Regulation of gene expressionby nuclear hormone receptors and their viral and oncogenicparalogs. 22. DESCRIPTION (Do notexceed space provided) In this work, I propose to characterizetwo isoforms of the nuclear receptor corepressor SMRT with respect to their ability ot interactwith various nuclear hormone receptors and their ability to effect transcriptional repression. I will also analyze the expression of these isoforms in differenttissue to determine if expressionof these isoforms are regulated and to potentially gain insight into the physiological roles these isoformsmay play. In addition, I will develop an algorithm which will use data from the EST database to identify other splicing isoforms of SMRT. PHS 416-1 (Rev. 12/98) Form Page 2 E 3 L Table of Contents ========================================Section End===========================================
| Goodson, Michael L; Farboud, Behnom; Privalsky, Martin L (2007) An improved high throughput protein-protein interaction assay for nuclear hormone receptors. Nucl Recept Signal 5:e002 |
| Goodson, Michael L; Jonas, Brian A; Privalsky, Martin L (2005) Alternative mRNA splicing of SMRT creates functional diversity by generating corepressor isoforms with different affinities for different nuclear receptors. J Biol Chem 280:7493-503 |
