Abstract

This research program on the protozoan parasite E. histolytica has focused on the isolation and characterization of surface antigens of amebae that interact with host intestinal cells, on understanding the immune response to E. histolytical infection, and developing recombinant antigen-based vaccines to prevent amebic disease. Many of these studies have utilized the serine rich E. histolytica protein (SREHP), a novel surface protein of amebae. The recent development of a scid mouse model of amebic infection, and the cloning and expression of 3 recombinant E. histolytica antigens to study protective immunity to amebic infection, and to develop vaccines to prevent amebic infection.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Modified Research Career Development Award (K04)
Project #
5K04AI001231-05
Application #
2671354
Study Section
Tropical Medicine and Parasitology Study Section (TMP)
Project Start
1994-09-01
Project End
1999-08-31
Budget Start
1998-09-01
Budget End
1999-08-31
Support Year
5
Fiscal Year
1998
Total Cost
Indirect Cost

  Institution

Name
Washington University
Department
Internal Medicine/Medicine
Type
Schools of Medicine
DUNS #
062761671
City
Saint Louis
State
MO
Country
United States
Zip Code
63130

  Publications

Stanley Jr, S L; Reed, S L (2001) Microbes and microbial toxins: paradigms for microbial-mucosal interactions. VI. Entamoeba histolytica: parasite-host interactions. Am J Physiol Gastrointest Liver Physiol 280:G1049-54
Stenson, W F; Zhang, Z; Riehl, T et al. (2001) Amebic infection in the human colon induces cyclooxygenase-2. Infect Immun 69:3382-8
Zhang, Z; Wang, L; Seydel, K B et al. (2000) Entamoeba histolytica cysteine proteinases with interleukin-1 beta converting enzyme (ICE) activity cause intestinal inflammation and tissue damage in amoebiasis. Mol Microbiol 37:542-8
Seydel, K B; Smith, S J; Stanley Jr, S L (2000) Innate immunity to amebic liver abscess is dependent on gamma interferon and nitric oxide in a murine model of disease. Infect Immun 68:400-2
Zhang, T; Stanley Jr, S L (1999) DNA vaccination with the serine rich Entamoeba histolytica protein (SREHP) prevents amebic liver abscess in rodent models of disease. Vaccine 18:868-74
Sultan, F; Jin, L L; Jobling, M G et al. (1998) Mucosal immunogenicity of a holotoxin-like molecule containing the serine-rich Entamoeba histolytica protein (SREHP) fused to the A2 domain of cholera toxin. Infect Immun 66:462-8
Seydel, K B; Zhang, T; Champion, G A et al. (1998) Cryptosporidium parvum infection of human intestinal xenografts in SCID mice induces production of human tumor necrosis factor alpha and interleukin-8. Infect Immun 66:2379-82
Stanley Jr, S L; Jackson, T F; Foster, L et al. (1998) Longitudinal study of the antibody response to recombinant Entamoeba histolytica antigens in patients with amebic liver abscess. Am J Trop Med Hyg 58:414-6
Seydel, K B; Li, E; Zhang, Z et al. (1998) Epithelial cell-initiated inflammation plays a crucial role in early tissue damage in amebic infection of human intestine. Gastroenterology 115:1446-53
Seydel, K B; Stanley Jr, S L (1998) Entamoeba histolytica induces host cell death in amebic liver abscess by a non-Fas-dependent, non-tumor necrosis factor alpha-dependent pathway of apoptosis. Infect Immun 66:2980-3

Showing the most recent 10 out of 22 publications