Abstract

In the context of the research proposal, a competitive PCR assay will be used to analyze the profiles of expression of the mRNAs encoding subunits of the GABA-A receptor during development (in vivo) and as cerebellar granule cells differentiate in vitro. This information will be correlated with a comparable in situ hybridization analysis during development in vivo. The hypothesis that cell type specific patterns of expression of the receptor subunit mRNAs are influenced by both heterologous and homologous receptor stimulation will be tested. In the context of, the mechanism by which this trans-synaptic regulation of GABA-A receptor subunit mRNAs occurs will be investigated.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Modified Research Career Development Award (K04)
Project #
5K04NS001647-04
Application #
2259657
Study Section
Neurology C Study Section (NEUC)
Project Start
1993-09-01
Project End
1998-08-31
Budget Start
1995-09-01
Budget End
1996-08-31
Support Year
4
Fiscal Year
1995
Total Cost
Indirect Cost

  Institution

Name
Allegheny-Singer Research Institute
Department
Type
DUNS #
033098401
City
Pittsburgh
State
PA
Country
United States
Zip Code
15212

  Publications

Shumsky, Jed S; Wu, Yunxing; Murphy, E Hazel et al. (2002) Differential effects of prenatal cocaine exposure on selected subunit mRNAs of the GABA(A) receptor in rabbit anterior cingulate cortex. J Chem Neuroanat 24:243-55
Lauder, J M; Liu, J; Grayson, D R (2000) In utero exposure to serotonergic drugs alters neonatal expression of 5-HT(1A) receptor transcripts: a quantitative RT-PCR study. Int J Dev Neurosci 18:171-6
Grayson, D R; Zhu, W; Harris, B T et al. (1998) Differentially expressed GABAA-receptor subunits result in structurally and functionally receptor assemblies following excitatory afferent synaptic transmission. Perspect Dev Neurobiol 5:193-205
Mizukami, K; Grayson, D R; Ikonomovic, M D et al. (1998) GABAA receptor beta 2 and beta 3 subunits mRNA in the hippocampal formation of aged human brain with Alzheimer-related neuropathology. Brain Res Mol Brain Res 56:268-72
Wang, X H; Zhu, W J; Corsi, L et al. (1998) Chronic dizocilpine (MK-801) reversibly delays GABA(A) receptor maturation in cerebellar granule neurons in vitro. J Neurochem 71:693-704
Shumsky, J S; Wu, Y; Murphy, E H et al. (1998) Prenatal cocaine exposure does not affect selected GABAA receptor subunit mRNA expression in rabbit visual cortex. Ann N Y Acad Sci 846:371-4
Vicini, S; Wang, J F; Li, J H et al. (1998) Functional and pharmacological differences between recombinant N-methyl-D-aspartate receptors. J Neurophysiol 79:555-66
Ikonomovic, S; Kharlamov, E; Manev, H et al. (1997) GABA and NMDA in the prevention of apoptotic-like cell death in vitro. Neurochem Int 31:283-90
Liu, J; Morrow, A L; Devaud, L L et al. (1997) Regulation of GABA(A) receptor subunit mRNA expression by the pesticide dieldrin in embryonic brainstem cultures: a quantitative, competitive reverse transcription-polymerase chain reaction study. J Neurosci Res 49:645-53
McCall, W D; Tanner, K D; Levine, J D (1996) Formalin induces biphasic activity in C-fibers in the rat. Neurosci Lett 208:45-8

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