In the context of the research proposal, a competitive PCR assay will be used to analyze the profiles of expression of the mRNAs encoding subunits of the GABA-A receptor during development (in vivo) and as cerebellar granule cells differentiate in vitro. This information will be correlated with a comparable in situ hybridization analysis during development in vivo. The hypothesis that cell type specific patterns of expression of the receptor subunit mRNAs are influenced by both heterologous and homologous receptor stimulation will be tested. In the context of, the mechanism by which this trans-synaptic regulation of GABA-A receptor subunit mRNAs occurs will be investigated.

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Modified Research Career Development Award (K04)
Project #
5K04NS001647-04
Application #
2259657
Study Section
Neurology C Study Section (NEUC)
Project Start
1993-09-01
Project End
1998-08-31
Budget Start
1995-09-01
Budget End
1996-08-31
Support Year
4
Fiscal Year
1995
Total Cost
Indirect Cost
Name
Allegheny-Singer Research Institute
Department
Type
DUNS #
033098401
City
Pittsburgh
State
PA
Country
United States
Zip Code
15212
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