The Wilms' tumor suppressor gene WT1 encodes a transcription factor of critical importance in early kidney development. This project will examine regulation of the human WT1 gene, using its promoter to identify cis regulatory DNA sequences and the corresponding transcriptional regulatory proteins that bind to them that are most important for WT1 expression in kidney development. This promoter analysis approach is well recognized in developmental biology and allows antecedent transcriptional events to be uncovered. Such studies may also shed light on embryonic kidney-,kidney epithelial-, and/or podocyte-specific gene expression, and thereby suggest alternative treatments, such as gene therapy, for acute and chronic glomerular disease.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Clinical Investigator Award (CIA) (K08)
Project #
1K08DK002280-01
Application #
2134148
Study Section
Diabetes, Endocrinology and Metabolic Diseases B Subcommittee (DDK)
Project Start
1994-04-20
Project End
1999-03-31
Budget Start
1994-04-20
Budget End
1995-03-31
Support Year
1
Fiscal Year
1994
Total Cost
Indirect Cost
Name
Beth Israel Deaconess Medical Center
Department
Type
DUNS #
076593722
City
Boston
State
MA
Country
United States
Zip Code
02215
Cohen, H T; Zhou, M; Welsh, A M et al. (1999) An important von Hippel-Lindau tumor suppressor domain mediates Sp1-binding and self-association. Biochem Biophys Res Commun 266:43-50
Knebelmann, B; Ananth, S; Cohen, H T et al. (1998) Transforming growth factor alpha is a target for the von Hippel-Lindau tumor suppressor. Cancer Res 58:226-31
Mukhopadhyay, D; Knebelmann, B; Cohen, H T et al. (1997) The von Hippel-Lindau tumor suppressor gene product interacts with Sp1 to repress vascular endothelial growth factor promoter activity. Mol Cell Biol 17:5629-39
Cohen, H T; Bossone, S A; Zhu, G et al. (1997) Sp1 is a critical regulator of the Wilms' tumor-1 gene. J Biol Chem 272:2901-13