Adenoviral vector-mediated gene transfer is particularly attractive for cells that are refractory to standard methods of transfection, such as calcium phosphate precipitation, liposomes or electroporation. This includes primary cell cultures and other highly differentiated cells, including non-dividing cells. For the same reasons, adenoviral vectors are the method of choice for in situ gene transfer. In addition, adenoviral vectors may be used to express several exogenous genes in a cell, either simultaneously or at defined intervals. Levels of gene expression can be controlled by the dose of virus used. The purpose of this project is to construct replication-deficient vectors for in vitro use. These vectors will direct the expression of a wide range of proteins that are of interest to investigators in the NIDR Division of Intramural Research. The vectors will be used to study signal transduction, extracellular matrix and salivary gland physiology. Specifically, the first goal of the contract will be to grow and purify plasmids containing adenovirus sequences. The second will be to recombine plasmids to generate adenovirus vectors. The third goal of the project will be to amplify isolated adenovirus plaques and to purify them into virus stocks. These reagents will be unique and not available from commercial sources.

Project Start
1997-06-01
Project End
2001-05-31
Budget Start
2000-03-07
Budget End
2001-05-31
Support Year
Fiscal Year
2000
Total Cost
Indirect Cost
Name
Microbiological Associates, Inc.
Department
Type
DUNS #
City
Rockville
State
MD
Country
United States
Zip Code
20850