This is a revised application seeking four years of funding to study structure-function relationships of the human erythropoietin receptor. The Principal Investigator has cloned the human erythropoietin receptor cDNA using the known murine sequence, and localized the gene to chromosome 19p. The goals of the project are broad, ranging from identification of ligand-binding sites of the external domain, analysis of the signal transducing properties of the cytoplasmic domain, analysis of receptor turnover, and studies of the regulation of the receptor gene in normal and leukemic erythroid cells.The investigator recognizes that similar work is being conducted by several groups using the murine receptor, but he correctly points out that studies on the human receptor are warranted in order to better understand human diseases such as polycythemia vera.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Research Project (R01)
Project #
5R01DK044134-04
Application #
2143544
Study Section
Hematology Subcommittee 2 (HEM)
Project Start
1992-05-01
Project End
1996-04-30
Budget Start
1994-05-10
Budget End
1995-04-30
Support Year
4
Fiscal Year
1994
Total Cost
Indirect Cost
Name
University of Cincinnati
Department
Internal Medicine/Medicine
Type
Schools of Medicine
DUNS #
City
Cincinnati
State
OH
Country
United States
Zip Code
45221
Ward, J C; Harris, K W; Penny, L A et al. (1992) A structurally abnormal erythropoietin receptor gene in a human erythroleukemia cell line. Exp Hematol 20:371-3
Winkelmann, J C (1992) The human erythropoietin receptor. Int J Cell Cloning 10:254-61