Abstract

The overall goals of this program remain directed towards a better understanding of cellular regulation. The individual components of the program are: 1. Bone marrow transplantation of HLA-matched and haploidentical patients. Specifically, bone marrows will be depleted of mature T cells by the use of anti-Leu 1 antibody plus complement. The engraftment, graft versus host disease and immune reconstitution of these patients will be evaluated. Animal models will be employed to study engraftment and graft vs. host disease. 2. The cell-cell interactions which regulate erythropoiesis and in particular hematopoiesis in human and murine systems will be studied. We have partially purified progenitor cells and we are currently studying the effect of cloned CSF-GM on their differentiation. Also the effects on hematopoiesis of stromal cell CSF is being investigated.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Program Projects (P01)
Project #
5P01CA018662-11
Application #
3092786
Study Section
(SRC)
Project Start
1978-09-01
Project End
1987-02-28
Budget Start
1986-03-01
Budget End
1987-02-28
Support Year
11
Fiscal Year
1986
Total Cost
Indirect Cost

  Institution

Name
Dana-Farber Cancer Institute
Department
Type
DUNS #
149617367
City
Boston
State
MA
Country
United States
Zip Code
02115

  Publications

Ferrara, J L; Mauch, P; Van Dijken, P J et al. (1990) Evidence that anti-asialo GM1 in vivo improves engraftment of T cell-depleted bone marrow in hybrid recipients. Transplantation 49:134-8
Blatter, D D; Crawford, J M; Ferrara, J L (1990) Nuclear magnetic resonance of hepatic graft-versus-host disease in mice. Transplantation 50:1011-8
van Dijken, P J; Ghayur, T; Mauch, P et al. (1990) Evidence that anti-LFA-1 in vivo improves engraftment and survival after allogeneic bone marrow transplantation. Transplantation 49:882-6
Ferrara, J L; Guillen, F J; van Dijken, P J et al. (1989) Evidence that large granular lymphocytes of donor origin mediate acute graft-versus-host disease. Transplantation 47:50-4
Bierer, B E; Burakoff, S J; Smith, B R (1989) A large proportion of T lymphocytes lack CD5 expression after bone marrow transplantation. Blood 73:1359-66
Ferrara, J L; Michaelson, J; Burakoff, S J et al. (1988) Engraftment following T cell-depleted bone marrow transplantation. III. Differential effects of increased total-body irradiation on semiallogeneic and allogeneic recipients. Transplantation 45:948-52
Bierer, B E; Emerson, S G; Antin, J et al. (1988) Regulation of cytotoxic T lymphocyte-mediated graft rejection following bone marrow transplantation. Transplantation 46:835-9
Bierer, B E; Nishimura, Y; Burakoff, S J et al. (1988) Phenotypic and functional characterization of human cytolytic T cells lacking expression of CD5. J Clin Invest 81:1390-7
Antin, J H; Ault, K A; Rappeport, J M et al. (1987) B lymphocyte reconstitution after human bone marrow transplantation. Leu-1 antigen defines a distinct population of B lymphocytes. J Clin Invest 80:325-32
Ferrara, J L; Mauch, P; McIntyre, J et al. (1987) Engraftment following T-cell-depleted bone marrow transplantation. II. Stability of mixed chimerism in semiallogeneic recipients after total-body irradiation. Transplantation 44:495-9

Showing the most recent 10 out of 28 publications