Abstract

The development of the humoral immune system will be evaluated in animal models where there is evidence for genetically-determined abnormalities. These include: autoimmune NZB, BXSB, and MRL/Lpr; immunodeficient CBA/N; autoinuune/immunodeficient motheaten; and stem cell deficient W/W mice. Cell separation, transfer, and culture methods as well as monoclonal antibodies to cell surface antigens are now available for identifying, enumerating, and manipulating several types of B lineage-precursor cells. Preliminary observations indicate that it may be possible to localize particular defects in these models and attribute them to differentiating B ormicroenvironmental cells. The studies will then be extended with investigations concerning the kinetics of formation of B cells and their precursors. The discovery of a conserved antigen shared by the lymphoid cells of mouse and man should make it feasible to extend certain of these findings to an analysis of patients with immunodeficiency or immunoregulatory diseases.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
5R01AI019884-04
Application #
3129339
Study Section
Immunobiology Study Section (IMB)
Project Start
1982-08-01
Project End
1987-07-31
Budget Start
1985-08-01
Budget End
1986-07-31
Support Year
4
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
Oklahoma Medical Research Foundation
Department
Type
DUNS #
937727907
City
Oklahoma City
State
OK
Country
United States
Zip Code
73104

  Publications

Jacobsen, K; Kravitz, J; Kincade, P W et al. (1996) Adhesion receptors on bone marrow stromal cells: in vivo expression of vascular cell adhesion molecule-1 by reticular cells and sinusoidal endothelium in normal and gamma-irradiated mice. Blood 87:73-82
Kincade, P W; Medina, K L; Smithson, G et al. (1994) Pregnancy: a clue to normal regulation of B lymphopoiesis. Immunol Today 15:539-44
Gimble, J M; Medina, K; Hudson, J et al. (1993) Modulation of lymphohematopoiesis in long-term cultures by gamma interferon: direct and indirect action on lymphoid and stromal cells. Exp Hematol 21:224-30
Lesley, J; Kincade, P W; Hyman, R (1993) Antibody-induced activation of the hyaluronan receptor function of CD44 requires multivalent binding by antibody. Eur J Immunol 23:1902-9
Pelletier, R; Ohye, R; Kincade, P et al. (1993) Monoclonal antibody to anti-VCAM-1 interferes with murine cardiac allograft rejection. Transplant Proc 25:839-41
Pelletier, R P; Morgan, C J; Sedmak, D D et al. (1993) Analysis of inflammatory endothelial changes, including VCAM-1 expression, in murine cardiac grafts. Transplantation 55:315-20
Lesley, J; Hyman, R; Kincade, P W (1993) CD44 and its interaction with extracellular matrix. Adv Immunol 54:271-335
Kincade, P W; He, Q; Ishihara, K et al. (1993) CD44 and other cell interaction molecules contributing to B lymphopoiesis. Curr Top Microbiol Immunol 184:215-22
Ishihara, K; Wood Jr, W J; Damore, M et al. (1992) B29 gene products complex with immunoglobulins on B lymphocytes. Proc Natl Acad Sci U S A 89:633-7
Hession, C; Moy, P; Tizard, R et al. (1992) Cloning of murine and rat vascular cell adhesion molecule-1. Biochem Biophys Res Commun 183:163-9

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