The development of the humoral immune system will be evaluated in animal models where there is evidence for genetically-determined abnormalities. These include: autoimmune NZB, BXSB, and MRL/Lpr; immunodeficient CBA/N; autoinuune/immunodeficient motheaten; and stem cell deficient W/W mice. Cell separation, transfer, and culture methods as well as monoclonal antibodies to cell surface antigens are now available for identifying, enumerating, and manipulating several types of B lineage-precursor cells. Preliminary observations indicate that it may be possible to localize particular defects in these models and attribute them to differentiating B ormicroenvironmental cells. The studies will then be extended with investigations concerning the kinetics of formation of B cells and their precursors. The discovery of a conserved antigen shared by the lymphoid cells of mouse and man should make it feasible to extend certain of these findings to an analysis of patients with immunodeficiency or immunoregulatory diseases.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
5R01AI019884-05
Application #
3129340
Study Section
Immunobiology Study Section (IMB)
Project Start
1982-08-01
Project End
1987-07-31
Budget Start
1986-08-01
Budget End
1987-07-31
Support Year
5
Fiscal Year
1986
Total Cost
Indirect Cost
Name
Oklahoma Medical Research Foundation
Department
Type
DUNS #
937727907
City
Oklahoma City
State
OK
Country
United States
Zip Code
73104
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Lesley, J; Hyman, R; Kincade, P W (1993) CD44 and its interaction with extracellular matrix. Adv Immunol 54:271-335
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